Figure 5

Immunostaining of primary Patient Tumor in comparison to xenografts of PaCa 5061 cells in pfp ^-/- /rag2 ^-/- mice. Primary patient tumor as wells as primary mouse tumor and corresponding lung metastasis were immunostained. 5A. Human primary tumor, mouse tumor and corresponding lung metastasis are positive for cytokeratin and pancreatic cancer cell markers CEA and CA 19-9 respectively. Positive immunostainings for overexpressed Mucin4 are shown, confirming the Microarray data. 5B. Patient primary tumor as well as mouse tumors are highly positive for EGF receptor as well as for CEACAM 5, and -6 adhesion molecules, confirming the Microarray data. Remarkably, the pattern of the staining intensity as well as the expression pattern showed a high level of similarity between human and mouse primary tumor.