Figure 8From: Inoculated mammary carcinoma-associated fibroblasts: contribution to hormone independent tumor growth PMN and mast cells at early stages (5 days) of tumor development. (A) Hematoxylin and eosin (upper panel) or toluidine blue (lower panel) staining of EPI-HI or EPI-HI+CAF-HI isografts. BALB/c mice were sc inoculated with CAF-HI and/or EPI-HI cells. Original magnification: 100× Bar: 72 μm. (Insets) Magnification showing (upper panel) PMN cells or (lower panel) mast cells within the inoculation site. (B) Tumor growth of purified EPI-HI (1.5 × 104 cells) alone or admixed with CAF-HI (1.5 × 104) implanted sc in NOD/SCID mice (n = 4-5). EPI-HI or EPI-HI+CAF-HI were prepared as described in Materials and Methods. ***: p < 0.001; **: p < 0.01; *: p < 0.05. Tumor size: Mean ± s.d. (Inset) Tumor weight at the end of the experiment (day 30) for EPI-HI and EPI-HI+CAF-HI tumors.Back to article page