Table 2 Primer sequences for PCR
From: Epigenetic alterations differ in phenotypically distinct human neuroblastoma cell lines
Gene | Purpose | Sense primer | Antisense primer |
|---|---|---|---|
BLU | Q-PCR | CACGAGGCCTCCATCATCA | ACTCACACACCTCCTTGTGGAA |
CASP8 | Q-PCR | TGCAGAGGGAACCTGGTACAT | TCGAGGACATCGCTCTCTCA |
GAPDH | Q-PCR | CCATGGGGAAGGTGAAGGTCGGACTC | GGTGGTGCAGGCATTGCTGATG |
HIC-1 | Q-PCR | TGCTGCAGCTCAACAACCA | GGCGTTCTGCACCACGAT |
HIN-1 | Q-PCR | CACCCTCAACCCGCTGAA | ACACTTCTGGGAGCCCTCTATG |
SPARC | Q-PCR | TCTTCCCTGTACACTGGCAGTTC | AGCTCGGTGTGGGAGAGGTA |
THBS1 | Q-PCR | TGGAACTATGGGCTTGAGAAAAC | CACTGATGCAAGCACAGAAAAGA |
TIG-1 | Q-PCR | CCTGGCAAACCTCTTAAAGTGAA | GGAGGCTTCTTCTGGTGTCTGT |
Actin | QMSP | TGGTGATGGAGGAGGTTTAGTAAGT | AACCAATAAAACCTACTCCTCCCTTAA |
BLU | QMSP | GTAGTTATGGAAACGGGTTAGTC | CGCTAAAAATCCAAATACTATAACG |
CASP8 | QMSP | AGGTAGGAGAATCGTTTGAATTC | AACGAAATTTCGCTCTTATTACC |
HIC-1 | QMSP | GTTAGGCGGTTAGGGCGTC | CCGAACGCCTCCATCGTAT |
HIN-1 | QMSP | AGGGACGATTAGGTTTTATTTTC | CTAATTTCGAAAACAAAAAAACG |
MYOD1 | QMSP | CCAACTCCAAATCCCCTCTCTAT | TGATTAATTTAGATTGGGTTTAGAGAAGGA |
SPARC | QMSP | GTTGTTATATTCGGGGACGAC | TAACCCGTTTCCATAACTACGA |
THBS1 | QMSP | GAAAGGGTTCGAAGGTAGC | CCGAACGCCTATCCTAAA |
TIG-1 | QMSP | GTTTAGTGTTAGGATGCGGTATC | AAAACGCGATACGAAATAACA |
THBS1 | ChIP-1 | ATGACAACTTGGCAAAAAAGAGAA | GGCCGAAGTGATGGACCTT |
THBS1 | ChIP-2 | AATGTGGGTGAATTCCTGTTAAGG | GGGTGGAAAGGAAAGGTCATAGA |
THBS1 | ChIP-3 | TGGGTGCCGATTCCAGAA | TCCCGCAAATCATAGGTAATGA |
THBS1 | ChIP-4 | CCACGCAAGAAAAGCGAAA | TGCTGCAAACAGCCAAGTG |
THBS1 | ChIP-5 | GGGCAGGTACTTTAACGAATGG | CTGGGCCCGTTTTGTAAAAA |
THBS1 | ChIP-6 | GGCGGGCACCGACTTT | GCGCAACTTTCCAGCTAGAAA |