Figure 5
From: Epigenetic alterations differ in phenotypically distinct human neuroblastoma cell lines
Alteration of histone marks around THBS-1 promoter region. A, ChIP/quantitative PCR was performed around THBS-1 promoter region in LA1-55n and VPA-treated LA1-55n cells. B, ChIP/quantitative PCR was performed around THBS-1 promoter region in 5-Aza-dC treated and non-treated LA1-55n cells. The enrichment of three histone marks related to repressive chromatin state is markedly reduced in 5-Aza-dC treated LA1-55n cells compared with untreated ones. C, ChIP assay was performed around THBS-1 promoter region in 5-Aza-dC treated and non-treated LA1-55n cells. D, Luciferase assay was performed to examine the promoter activity of THBS-1 between LA1-55n and LA1-5s cells.