Figure 5

Qct causes changes in p73 distribution. A) Non-quantitative PCR in DB-1 cells for TAp73 (Lane 1), ΔNp73 (Lane 2) and TAp73 positive control using SK Mel 28 cells (Lane 3) following treatment with TMZ. TAp73 was undetectable in the DB-1 cell line. B) Real time RT-PCR for ΔNp73 in DB-1 cell lines treated with TMZ 400 μM for 48 hrs followed Qct 75 μM for 24 hrs. Results are Mean ± SEM of triplicate experiments in DB-1 cell lines. C) p73 expression by western blot analysis in DB-1 and SK Mel 28 cell lines treated with vehicle (DMSO), TMZ 400 μM for 48 hrs followed by Qct 75 μM for 24 hrs. D) Immunocytochemical analysis of p73 localization in DB-1 cell lines treated with vehicle (DMSO), TMZ 400 μM for 48 hrs followed by Qct 75 μM for 24 hrs. Solid arrowheads indicate nuclear staining in cells without Qct treatment, while line-type arrowheads indicate cytoplasmic staining following Qct treatment.