Figure 7

Effects of miR-21 inhibitor and taxol on cell invasion ability in U251 and LN229 cell lines. Cell invasion ability was assessed by a transwell assay after 48 h treatment with the miR-21 inhibitor and taxol, alone or in combination (Fig. 7A). The numbers of cells that could invade via the membrane are shown as a histogram (Fig. 7B). Western blotting validation of proteins (MMP-2 and MMP-9) downregulated by miR-21 inhibitor and taxol combination therapy in both U251 and LN229 cell lines (Fig. 7C). The expression of β-actin was examined to ensure uniform protein loading in all lanes.