Figure 6

Effect of BITC and PEITC on metastasis-related gene expression. (A) L9981 cells were treated with 5 μM of BITC or 10 μM of PEITC for 4 h. MMP-2, Twist and β-catenin mRNA expressions were detected by real-time PCR. (B) L9981 cells were treated with 5 - 20 μM of BITC or PEITC for 24 h. MMP-2 and Twist protein expression were detected by Western blotting analyses (* P < 0.05, ** P < 0.01, *** P < 0.001). Similar results were obtained in three independent experiments.