Figure 8
Characterization of epithelial structures in mixed Matrigel™-collagen gels after two weeks in culture. Co-cultures of MCF7 cells and RMF were characterized using immunohistochemical analyses for luminal epithelial cell origin (Keratin 18), adherens junctions (E-cadherin), and polarization (Sialomucin). No staining was observed for the basement membrane marker type IV collagen. Arrow points to the luminal localization of Sialomucin staining; arrowhead signals fibroblast. Scale bar: 50 μm.