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Table 3 Assessment of siS100A4 specificity.

From: Overexpression of S100A4 in human cancer cell lines resistant to methotrexate

Gene

S100A4

Enolase 2

Topoisomerase II

Clusterin

UGT1A7

Control

100

100

100

100

100

siRNA S100A4

18.6 ± 6

135.8 ± 21.6

127.5 ± 6.4

133.9 ± 14.4

159.5 ± 28.4

  1. HT29 cells were transfected with 100 nM siS100A4. Forty-eight hours later, the mRNA levels of S100A4 and four unrelated genes were determined by RT-Real-Time PCR. Data represent the mean of three experiments ± SE.