Figure 2From: Signal transduction mechanisms involved in S100A4-induced activation of the transcription factor NF-κB Dose dependent reduction in phosphorylated IκBα and total IκBα in S100A4-stimulated cells by Ser/Thr kinase inhibitors. II-11b cells were treated with 2 μM S100A4 and the inhibitors H-7 (A) and staurosporine (B) using the indicated concentrations. After one hour cells were harvested and analyzed by immunoblotting for expression of phosphorylated IκBα, total IκBα and α-tubulin as indicated. For total IκBα both short and long exposure times are shown.Back to article page