Figure 1

Dose dependent reduction in phosphorylation of IκBα by Ser/Thr kinase inhibitors. II-11b cells were pretreated with the inhibitors H-7 (A), staurosporine (B), U-73122 (C) compound 48/80 (D), AG 18 (E), genistein (F), GF 109203X (G), GDPβ (H), suramin (I) or LY294002 (J) using the indicated concentrations. Thereafter, cells were stimulated with 2 μM S100A4 for one hour, harvested and analyzed by immunoblotting for expression of phosphorylated IκBα and α-tubulin. The figures shown are representative of two or three independent experiments.