Identification of valid reference genes for gene expression studies of human stomach cancer by reverse transcription-qPCR

Table 4 Ranking of the candidate single reference genes based on their stability values calculated from NormFinder.

Non-stomach cancer cell lines		Stomach cancer cell lines		Normal stomach tissues		Tumor stomach tissues		All stomach tissues		All stomach cell lines + tissues
Gene in ranking order	Stability value	Gene in ranking order	Stability value	Gene in ranking order	Stability value	Gene in ranking order	Stability value	Gene in ranking order	Stability value	Gene in ranking order	Stability value
GAPDH	0.036	B2M	0.014	RPL29	0.028	RPL29	0.028	RPL29	0.032	ACTB	0.029
RPL29	0.052	GAPDH	0.021	B2M	0.035	B2M	0.039	B2M	0.041	HPRT1	0.038
B2M	0.053	RPL29	0.029	HPRT1	0.038	HPRT1	0.042	HPRT1	0.044	RPL29	0.068
HPRT1	0.110	18S rRNA	0.036	ACTB	0.043	ACTB	0.065	ACTB	0.052	18S rRNA	0.071
18S rRNA	0.112	ACTB	0.060	18S rRNA	0.062	18S rRNA	0.067	18S rRNA	0.055	GAPDH	0.082
ACTB	0.143	HPRT1	0.115	GAPDH	0.140	GAPDH	0.147	GAPDH	0.140	B2M	0.084

ISSN: 1471-2407