Figure 6

Effect of IGF-1R siRNA (A), and resveratrol (B) on IGF-1R/Wnt signaling pathway proteins. A - HT-29 cells were treated with DMSO (control), non specific siRNA (siRNA control; 100 nM), positive siRNA (GAPDH siRNA; 100 nM) and IGF-1R siRNA (50, 75 and 100 nM) for 72 h. B - HT-29 cells were treated with DMSO, IGF-1 (10 nM) and/or resveratrol (150 μM) for 24 h. In case of both A and B, whole cell lysates were analyzed by western blotting as described in materials and methods. Proteins were detected by using specific antibodies. Band intensities normalized to loading control were indicated on top of the respective band (A). Similar results were obtained in duplicate experiments.