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Figure 3 | BMC Cancer

Figure 3

From: Aqueous Cinnamon Extract (ACE-c) from the bark of Cinnamomum cassiacauses apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential

Figure 3

Cinnamon decreased the expression of Her-2 oncoprotein. (A) Western blot analysis showing decrease in Her-2 expression in SiHa cells treated with different concentrations of ACE-c (0-80 μg/ml). Equal amounts of protein were loaded on 10% SDS-gel and immunoblotted with anti-Her-2 antibody. Tubulin was used as a loading control. Densitometric analysis of Her-2 expression was performed using phosphorimager. The data represents mean ± SD of five independent experiments. (B) Confocal images of the cells treated with indicated concentrations of ACE-c showing decrease in Her-2 expression. The cells were stained indirectly for Her-2 using Cy3 antibody (Panel II) and counterstained with DAPI (Panel I). Panel III represents the merge images.

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BMC Cancer

ISSN: 1471-2407

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