Figure 2

Cinnamon reduces migration potential.(A) Photomicrographs of time-lapse image at 0 and 15 h in a wound-healing assay in cells treated with different concentrations (0-80 μg/ml) of ACE-c. The upper panel of the image shows the wound made at 0 h. The lower panel shows cell movement corresponding to the distance traveled by the cells at 15 h of time-lapse imaging. (B) Rate of migration of cells during the wound healing assay analyzed by the time-lapse imaging of SiHa cells. Migration rate (μm/h) for each sample from five different fields was calculated. Error bars represent standard deviation and the data is representative of five independent experiments. (C) ACE-c treatment reduces the MMP-2 expression at mRNA level that has been shown by RT-PCR. β-actin was used as the loading control. Densitometric analysis of MMP-2 expression was performed using phosphorimager. The data represents mean ± SD of five independent experiments. (D) Gelatin zymography showing downregulation of MMP-2 expression in SiHa cells at 80 μg/ml ACE-c treatment compared to the untreated control cells. The bands were quantified by densitometry using phosphorimager and the data represents mean ± SD of five independent experiments.