Figure 1

RNA interference in prostate cancer cell lines. (A) DU145 and PC-3 cells were transfected with non-targeting control (NT) or anti-CSB (CSB) siRNA and CSB levels were assessed by immunoblot analysis of nuclear lysates. CSB and Ku86 migrated with apparent molecular weights of approximately 175 and 85 kDa, respectively. (B) Host cell reactivation of a UV-damaged reporter gene was assessed in DU145 and PC-3 cells transfected with non-targeting control (NT) or anti-CSB (CSB) siRNA. (C) The data in (B) is expressed as the dose required to reduce β-galactosidase activity by 50%. (D) The indicated cell lines were transfected with control (NT- closed symbols) or CSB targeting (open symbols) siRNA. Apoptosis was assessed as the proportion of cells with sub-diploid DNA content 48 hours following exposure to the indicated dose of cisplatin. Each value in B-D represents the mean (± SEM) from at least 4 independent experiments. An * in C indicates that the value is significantly less than 100% (P ≤ 0.05, single sample t-test) while an * in D indicates that the value is significantly different than its respective NT control (P ≤ 0.05, t-test). Similar results were obtained following exposure to UV light (data not shown).