Figure 4

Overexpression of Grp78 up-regulates FAK activation. (A), Western blot of phosphorylated Tyr 397 and Tyr 576/577 from serum starved mock-transfected cells and Grp78-transfected cells plated on Fibronectin for 1 hour. Corresponding total FAK is shown in the lower blot. (B), Western blot of phosphorylated Tyr 397 and Tyr 576/577 from serum starved Grp78 siRNA and control siRNA transfected Grp78 overexpressing cells plated on Fibronectin for 1 hour. (C), Representative image of a transwell assay using FAK siRNA and control siRNA transfected Grp78 overexpressing cells for investigating the effect of FAK siRNA knockdown in Grp78 overexpressing cells on Grp78 mediated tumor cells invasion. The experiments were repeated for three times in triplicate.