Table 1 Sequence of forward (F) and reverse primers (R) of indicated target genes and the size expected for each PCR-amplified product.
Peptidase | Gene symbol | Fordward primer | Reverse primer | Amplicon size (bp) |
|---|---|---|---|---|
DPPIV/gp110 | DPP4 | 5'-AGTGGCGTGTTCAAGTGTGG-3' | 5'-CAAGGTTGTCTTCTGGAGTTGG-3' | 112 |
NEP/CALLA glycoprotein | MME | 5'-CCGAGAAAAGGTGGACAAAGA-3' | 5'-GGACTGCTGGGCACTAAAGAA-3' | 133 |
Housekeeping Gene name | ||||
β-actin | ACTE | 5'-TCCCTGGAGAAGAGCTACGA-3' | 5'-ATCTGCTGGAAGGTGGACAG-3' | 362 |
Succinate dehydrogenase complex, subunit A | SDHA | 5'-TCTGCCCACACCAGCACT-3' | 5'-CCTCTCCACGACATCCTTCC-3' | 142 |
TATA box binding protein | TBP | 5'-GGATAAGAGAGCCACGAACCAC-3' | 5'-TTAGCTGGAAAACCCAACTTCTG-3' | 139 |
Peptidylpropyl isomerise A | PPIA | 5'-GGTCCCAAAGACAGCAGAAAA-3' | 5'-TCACCACCCTGACACATAAACC-3' | 114 |