Figure 5
THL inhibits the tube-formation ability of endothelial cells. A) THL dose-dependently inhibits FBS-induced capillary-like tube formation by HMEC-1 and HUVEC cells. Upper panel, images of FBS-induced tube formation by HMEC-1 cells in the presence of various concentrations of THL. Lower panel, quantitation of FBS-induced tube formation by HMEC-1 and HUVEC cells in the presence of various concentrations of THL. The number of tubes was counted in six randomly selected microscopic fields (× 200 magnification). The relative tube number was obtained by comparing the number of tubes formed in the presence of THL with that formed in the absence of THL. Values represent means ± SD, n = 2. *P < 0.05 versus untreated control. B) THL dose-dependently inhibits 231-CM-induced capillary-like tube formation by HMEC-1 cells. Upper panel, images of tube formation by HMEC-1 cells in various conditions. Lower panel, quantitation of tube formation by HMEC-1 cells in various conditions. The number of tubes was counted at 5 h post-seeding in six randomly selected microscopic fields (× 200 magnification). The relative tube number was obtained by comparing the number of tubes formed in different conditions with that formed in the presence of 231-CM without THL. Values represent means ± SD, n = 2. # P < 0.05 versus unstimulated control (in the absence of 231-CM and THL); **P < 0.001 versus 231-CM control (in the presence of 231-CM only).