Figure 4

VEGF-C activated moesin through RhoA/ROCK-2 pathway. (A-B) SiHa cells were treated with VEGF-C (100 ng/mL) for 48 h in the presence or absence of the ROCK-2 inhibitor, Y-27632 (Y - 10 μM) and moesin and phosphorylated moesin were assayed with western analysis. β-actin was used as the loading control. ** = P < 0.01 vs. corresponding control; ## = P < 0.01 vs VEGF-C 48 h. (C-D) SiHa cells were either mock-transfected (-Mock) or exposed to constitutively active or dominant-negative RhoA (- RhoA CA or - RhoA DN). Cells were then treated with VEGF-C (100 ng/mL) for 48 h and wild type and P-moesin were analyzed. ** = P < 0.01 vs. mock-transfected control; ## = P < 0.01 vs VEGF-C in mock or RhoA CA transfected group, or RhoA CA transfected alone. † = P < 0.05 vs. total RhoA in mock control; †† = P < 0.01 vs. total RhoA in mock control. All the above experiments were performed in triplicates and representative images are shown.