Normal and malignant epithelial cells with stem-like properties have an extended G2 cell cycle phase that is associated with apoptotic resistance

Table 2 Apoptotic and cell cycle data for cells derived from head and neck human oral carcinomas and from normal human oral mucosa.

Cell type	Treatment	%CD44 ^high	% Annexin positive cells in CD44 ^low	% Annexin positive cells in CD44 ^high	% cells in G2 in CD44 ^low	% cells in G2 in CD44 ^high
HNSCC derived cells (n = 11)
	*Ctrl 24 h*	3.9 ± 0.4	15.1 ± 11.5	8.9 ± 8.9	12.9 ± 1.2	23.0 ± 2.2^##
	*250 μM CP 24 h*	6.8 ± 1.0*	38.5 ± 19.5	22.1 ± 16.7	15.0 ± 2.1	41.1 ± 9.7^#
	*20 ng/ml NCZ 24 h*	6.0 ± 0.8	19.9 ± 10.3	3.5 ± 2.8	11.6 ± 1.9	31.4 ± 6.0^#
	*Ctrl 48 h*	4.3 ± 0.7	14.9 ± 7.6	2.5 ± 1.6	14.1 ± 3.1	23.5 ± 5.3
	*20 ng/ml NCZ48 h*	11.0 ± 3.9**	36.3 ± 16.3	11.0 ± 4.8	17.1 ± 2.9	76.9 ± 23.2**
*NOK* *(n = 5)*
	*Ctrl 24 h*	4.8 ± 1.7	5.2 ± 0.9	0.7 ± 0.4^##	10.4 ± 1.9	20.9 ± 2.6^#
	*10mJ UV 24 h*	7.5 ± 2.8	7.5 ± 2.8	1.3 ± 0.5^#	28.9 ± 2.1*	44.9 ± 4.7^##
	*Ctrl 48 h*	3.3 ± 0.6	15 ± 4.5	2.1 ± 0.3^#	11.6 ± 1.8	16.0 ± 2.3
	*250 μM CP 48 h*	4.6 ± 0.9	28.8 ± 4.0	3.6 ± 0.5* ^##	15.8 ± 4.0	28.6 ± 2.3

Table key: Data are presented as mean ± SD of 3 independent assays. * p value < 0.05 when compared with the control group; ** p value < 0.01 when compared with the control group; # p value < 0.05 when compared with the CD44 low population from the same group; ## p value < 0.01 when compared with the CD44 low population from the same group.

ISSN: 1471-2407