Figure 2
From: Lack of evidence for KRAS oncogenic mutations in triple-negative breast cancer
Detection of KRAS mutations by RT-PCR. A, The graph shows a representative amplification curve (ΔRn vs cycle) from 100 ng of genomic DNA prepared from a triple-negative tumor sample. RT-PCR reactions were performed with primers specifically designed to amplify wild-type KRAS (red) or the following mutants: Gly12Ala (green), Gly12Asp (blue), Gly12Arg (yellow), Gly12Cys (Pink), Gly12Ser (brown), Gly12Val (purple), Gly13Asp (grey). Brown lines correspond to the amplification profile of an internal control included in each reaction to check for false positives. B, As a positive control, genomic DNA was obtained from a colon carcinoma biopsy and subjected to RT-PCR as in A. Note the presence of the Gly12Cys mutation.