Figure 5

Effect of TBCC on different tubulins content and on subcellular tubulin fractions. (A) Western blot analysis of total cell extracts of the MC+1, MC+2, MC+3, MP6.1, MP6.2 and MP6.3 clones showing the effect of overexpression of TBCC on the expression level of α-tubulin, β-tubulin, tyrosinated α-tubulin, detyrosinated (glu) α-tubulin, β III-tubulin and acetylated α-tubulin. β-actin was used as a reference protein. (B) Representative blots corresponding to the expression levels of α-tubulin and β-tubulin in nonpolymerizable tubulin heterodimers (NPT), polymerizable tubulin (PT) heterodimers, microtubule heterodimers (MT) and total pool of tubulins in MC+1 and MP6.1 cells. These fractions were obtained after series of ultracentrifugations. (C) Protein ratios (MC+1 vs. MP6.1) of both α-tubulins and β-tubulins levels in NPT, PT and MT fractions. Results presented are the average values of three experiments. Bars represent standard deviation.