Figure 2

Effect of TBCC on proliferation rate, cell cycle distribution and mitotic duration of MC+1 and MP6.1 cells. (A) Optical density of absorbances measured at 540 and 690 nm of solubilized formazan crystals in MC+1 and MP6.1 formed after addition of MTT. The figure below the graph represents the MC+1 and MP6.1-containing wells colored with formazan crystals after addition of methylthiazoletetrazolium (MTT) every day for a total duration of one week. Results presented are the average values of three experiments. *: Values differ significantly from MP6.1 at ≥ 95% confidence level by Student's t-test. Bars represent standard deviation. (B) Proliferative capacity (BrdU labelling) of MC+1 and MP6.1 cells for one week. Results presented are the average values of three experiments. Bars represent standard deviation. (C) Cell cycle distribution of MC+1 and MP6.1 cells after incubation with propidium iodide. Percentage of cells in the S- and G2-M phase of the cell cycle in MC+1, MP6.1, MC+1 + siRNA SCR, MC+1 + siRNA TBCC, MP6.1 + siRNA SCR and MP6.1 + siRNA TBCC. The cells represented as +siRNA SCR and +siRNA TBCC are cells transiently double transfected with siRNA scrambled and siRNA targeting TBCC, respectively. Experiment was done 48 hours after first transfection. Results presented are the average values of three experiments. *: Values differ significantly at ≥ 95% confidence level by Student's t-test. Bars represent standard deviation. (D) Durations of prophase-metaphase, anaphase-telophase and complete mitosis in MC+1 and MP6.1 cells in minutes after 24 hours of time-lapse microscopic analysis. *: Values differ significantly from MP6.1 at ≥ 95% confidence level by Student's t-test. Bars represent standard deviation.