Figure 3

Immunohistochemical staining against ACE in the sham (B), control and captopril-treated animals at day 16 (C) and 21 (D). No staining was evident in the negative control (A). The sinusoidal, portal vein, and central vein endothelium stained positively for ACE in the sham liver (B), and two images at higher magnification on the right). At day 16, there appeared to be more positive staining in control tumors compared to treated tumors (C). In contrast, at day 21 more cells stained positively for ACE when treated with captopril than when untreated (D).