Validation of target CALM2 and CD9 mRNAs by quantitative RT-PCR. Quantitative RT-PCR using cell lysates, HuR antibody, and IgG1 from RIP-CHIP analysis confirmed results identifying CALM2 mRNA (A) and CD9 mRNA (B) as HuR targets. Change in gene expression is represented as fold increase in HuR immunoprecipitation as compared to IgG1. GAPDH mRNA was used as an endogenous control. Error bars represent SEM. p value is < 0.005. Experiments were done in triplicate (n = 3).