Figure 2

Suppressed TPM3 expression after siTPM3 transfection into HepG2 (A) and SNU-475 (B). TPM3 expression was measured by real-time qRT-PCR (top plots of A and B). X axis represents samples and Y axis relative TPM3 expression ratio (siTPM or siNEG/control). Error bars represent mean ± standard error of mean. Human GAPDH gene was used as internal control. TPM3 expression was also measured by western blot (bottom plots of A and B). TPM3 band intensities of siTPM-treated cells are much weaker than those of siNEG-treated ones and control, while internal control bands are consistent. Alpha-tubulin was used as internal control for western blot analysis. siTPM3, siTPM3 transfected HCC cell line; siNEG, negative oligonucleotide (siNEG) transfected HCC cell line; control, HCC cell line without transfection.