Combined analysis of cell growth and apoptosis-regulating proteins in HPVs associated anogenital tumors

BMC Cancer

Table 1 Sequences of specific probes and primers for real time RT-PCR

Gene	Application	Sequence	Length (bp)	5'	3'	Tm (°)	Genebank accession number
GAPDH	P	AAC AGC GAC ACC CAC TCC TCC ACC	24	FAM	BHQ1	67.4	NM_002046
	F	ACC AGG TGG TCT CCT CTG AC	20			58.5
	R	TGT AGC CAA ATT CGT TGT CAT ACC	24			58.6
c-IAP1	P	AGG AAA TGC TGC GGC CAA CAT CTT CA	26	ROX	BHQ2	66.8	NM_001166
	F	GAG AGA ACT GAT TGA TAC CAT TTT GG	26			57.6
	R	AGA CCT GAA ACA TCT TCT GTT GG	23			57.3
c-IAP2	P	TCC GTC AAG TTC AAG CCA GTT ACC CTC A	28	Cy5	BHQ3	67.2	NM_001165
	F	CTT GAT AAG AAT TAA AGG ACA GGA GTT C	28			58.0
	R	TGG GCT GTC TGA TGT GGA TAG	21			57.5
XIAP	P	CAG TGA AGA CCC TTG GGA ACA ACA TGC T	28	HEX	BHQ1	66.7	NM_001167
	F	GGA GGA GGG CTA ACT GAT TGG	21			58.1
	R	TAT TCT TGT CCC TTC TGT TCT AAC AG	26			57.9
c-Myc	P	CCA CCA CCA GCA GCG ACT CTG AGG A	25	HEX	BHQ1	69.0	NM_002467
	F	GGT GCT CCA TGA GGA GAC AC	20			58.1
	R	CCA GCA GAA GGT GAT CCA GAC	21			58.7

ISSN: 1471-2407