Expression of miR-21- target genes in male breast cancer in comparison to the control samples (A), correlation of miR-21 expression with the mRNA level of MASPIN (B) and PDCD4 (C) in male breast cancer specimens. Relative expression levels for every sample were calculated by normalizing to the mean expression level of two reference genes (βGUS and TBP). For every target gene the mean of the expression level in the control group was set equal to 1. Every individual measurement in the tumor samples was then normalized to this expression level. All measurements were performed independently two times. Error bars indicate standard deviation of the mean.