Figure 4From: In situ protein expression in tumour spheres: development of an immunostaining protocol for confocal microscopy CD44 and ALDH1 expression in HT29 spheroids. (A) HT29 spheroids were immunostained against CD44 (yellow, upper panel) or ALDH1 (yellow, lower panel) and nuclei were counterstained with TOPRO-3 (cyan). Acquisitions were taken at 70 μm depth (scale bar = 50 μm). All cells expressed CD44 while a large population of cells (86%) displayed ALDH1 staining. (B) Conventional pIHC using Cytoblock embedding technique confirmed that all cells were CD44+ and 85% were ALDH1+. (C) All cells from dissociated HT29 spheroids were found CD44+ in FC analysis.Back to article page