Figure 6
From: Immunodetection of nmt55/p54nrbisoforms in human breast cancer
Detection of nmt55/p54nrb protein isoforms by Western Blot Analysis using NMT-4, NMT-5 and NMT-1 antibodies. MCF-7 cell nuclear extract (50 μg), lane M, and ten different human breast tumor nuclear extracts (50 μg), lanes 1–10, were separated by SDS/PAGE and subjected to Western blot analysis with polyclonal antibody NMT-4 (Top Panel), polyclonal antibody NMT-5 (Middle Panel) and monoclonal antibody NMT-1 (Bottom Panel). Estrogen receptor (ER) expression levels were determined by ligand binding assay and are indicated along the top of the figure.