Imimunoprecipitation and immunodetection of nmt55/p54nrb protein with specific site-directed antibodies raised against various domains of nmt55/p54nrb. MCF-7 cell nuclear extracts (50 μg) were subjected to immunoprecipitation assays with the antibodies indicated along the top margin. Lane 1 represents immunoprecipitation with monoclonal antibody EVG F9 (raised against human estrogen receptor alpha), lane 2 monoclonal antibody NMT-1, lane 3 polyclonal antibody NMT-4 and lane 4 polyclonal antibody NMT-5. Immunoprecipitated samples were separated by SDS/PAGE and analyzed by Western blot. Left margin indicates the antibodies used for immunodetection. Top panel represents immunodetection with monoclonal antibody NMT-1, middle panel polyclonal antibody NMT-4 and bottom panel polyclonal antibody NMT-5.