Figure 4
From: Immunodetection of nmt55/p54nrbisoforms in human breast cancer
Assessment of NMT-4 and NMT-5 polyclonal antibody specificity for nmt55/p54nrb. MCF-7 nuclear extract (50 μg) was separated by SDS/PAGE and subjected to Western blot analysis. Lanes 1 and 2 represent immunodetection with NMT-4 pre-immune serum and NMT-5 pre-immune serum, respectively. Lanes 3 and 5 represent immunodetection with polyclonal antibodies NMT-4 and NMT-5, respectively. Lane 4 represents MCF-7 nuclear extract immunoblotted with polyclonal antibody NMT-4 pre-incubated with its corresponding immunogenic peptide. Lane 6 represents MCF-7 nuclear extract immunoblotted with polyclonal antibody NMT-5 pre-incubated with its corresponding immunogenic peptide. Right hand margin represents estimated molecular weight.