CD73 promotes proliferation and migration of human cervical cancer cells independent of its enzyme activity
- Zhao-wei Gao†1,
- Hui-ping Wang†1,
- Fang Lin1,
- Xi Wang1,
- Min Long1,
- Hui-zhong Zhang1Email author and
- Ke Dong1Email author
© The Author(s). 2017
Received: 16 January 2016
Accepted: 8 February 2017
Published: 15 February 2017
CD73 has both enzymatic and non-enzymatic functions in cells. As a nucleotidase, CD73 plays its enzymatic function by catalyzing the hydrolysis of AMP into adenosine and phosphate. In addition to this, accumulating data have shown that CD73 is a key regulatory molecule involved in cancer growth and metastasis, but this non-enzymatic function of CD73 in cervical cancer cells has not been well studied.
CD73 was overexpressed by pcDNA-NT5E expression vector transfection in Hela and SiHa cells. Cell’s proliferation and migration were evaluated by MTT and scratch healing assay. The CD73 specific antagonist -APCP was used to inhibit CD73 enzymatic activity. And the effect of APCP on CD73 activity was determined by high performance liquid chromatography (HPLC). Expression level was assessed by qRT-PCR and western blotting.
In the present study, we used Hela and SiHa cell lines to evaluate the effects of CD73 on cervical cancer cells proliferation and migration, and further explore the potential regulating mechanisms. Our data showed that CD73 overexpression significantly promoted cervical cancer cells proliferation and migration, and this promotive effect was not reverted by blocking CD73 enzymatic activity, both in Hela and SiHa cells. On the other hand, our data also showed that high concentration of adenosine inhibited Hela and SiHa cells proliferation and migration. These results demonstrated that the promotive effect of CD73 on cervical cancer cells proliferation and migration in vitro was independent from its enzymatic activity (i.e. production of adenosine). Furthermore, the expressions of EGFR, VEGF and Akt were significantly increased in CD73 overexpression Hela and SiHa cells.
Our data suggested that CD73 might promote proliferation and migration via potentiating EGFR/Akt and VEGF/Akt pathway, which was independent of CD73 enzyme activity. These data provide a novel insight into the regulating function of CD73 in cancer cells and suggest that CD73 may be promising therapeutic target in cervical cancer.
KeywordsCD73 Cervical cancer Proliferation Migration Enzyme activity
CD73 is a glycosylphosphatidylinositol (GPI) anchored cell surface protein, also known as ecto-5’-nucleotidase (ecto-5’-NT, EC 22.214.171.124). Accumulating data have shown that CD73 play important roles during cancer growth and metastasis [1–3]. CD73 has both enzymatic and non-enzymatic functions . As an enzyme, CD73 catalyzes the AMP breakdown into adenosine. Notably, adenosine is an important purine signaling molecule which has been found to be involved in tumor immunoescape . In addition to its enzymatic function, CD73 is also a regulatory molecule which related to cancer invasive and metastatic properties [3, 6, 7]. Studies have found that CD73 can promote proliferation and migration of several types of cancer cells [6, 8–10]. Cervical cancer, a cancer arising from cervix is due to the abnormal proliferation of cells that have the ability to evade growth suppression. Cervical cancer is high in the rank of cancers affect women, with both the fourth-highest incidence and the fourth-highest fatality rate among women worldwide . Infection with several special types of human papilloma virus (HPV) has been found to be the most high-risk cause of cervical cancer . However, HPV infection was not enough to trigger cervical cancer. Other factors, like molecular alteration have also been found to play important role during cervical cancer development [13, 14]. The role of CD73 in cervical cancer cells has not been well studied. In the present study, we investigated the effect of CD73 overexpression on cervical cancer cells proliferation and migration, and further explored its underlying regulatory mechanisms. Our data demonstrated that CD73 overexpression promoted Hela and SiHa cells proliferation and migration. Moreover, this promotive effect of CD73 should be via an enzymatic activity independent mechanism.
Two human cervical cancer cell lines, Hela and SiHa (American Type Culture Collection, ATCC), were used in this study. The cells were cultured in DMEM medium (Gibco, Carlsbad, NY, USA) supplemented with 10% heat-inactivated Fetal bovine serum (FBS, Sijiqing Biotec, Hangzhou, China) at 37 °C with 5% CO2 in a humidified incubator.
pcDNA-NT5E recombinant expression vector construction and transfection
CD73 coding gene - NT5E was cloned into the pcDNA3.0+ expression vector, then, the pcDNA-NT5E and control plasmids were transfected into Hela and SiHa cells using Lipofecta-mine™ 2000 reagent (Invitrogen, Carlsbad CA, USA) following the protocol offered by the manufacturer. Briefly, parental Hela and SiHa cells (3 × 105/well) were plated in six - well plate and cultured until 70 – 80% confluence. Premixed lipofection and plasmid DNA (10 μl : 4 μg) were added into the wells and incubated for 24 h. And then, the transfected cells were screened by G418.
CD73 activity assay
The conversion of AMP to adenosine was measured to assess the CD73 activity. Cultured cells were collected and suspended in DHank's buffer at a density of 4 × 105 cells/ml. 500 μl cell suspension was incubated with 1 mM AMP at 37 °C for 1 h, the effect of APCP on CD73 activity was investigated by adding 50 μM APCP into reaction system. Subsequently, cells were centrifuged, and the supernatants (10 μl) were subjected to HPLC analysis (Shimadzu, LC-2030c; column: GL Sciences, C18, 3.5 μm, 2.5 × 250 mm). Adenosine was extracted through a 0-50% methanol/H2O phase gradient elution (1 ml/min). The absorbance was measured at 259 nm. The peak volume (PV) for adenosine was calculated using LabSolutions LC Workstation Ver.5.
MTT cell viability assay
The cells were seeded in 96 - well plate at a density of 4 × 103 cells per well and cultured at 37 °C in DMEM medium plus 10% FBS. At different time point, 10 μl of MTT (5 mg/ml, Amresco, Solon, USA) was added into each well and the cells were incubated for another 4 h at 37 °C. The supernatants were then discarded carefully and 150 μl DMSO was added to each well. The plate was shaken for additional 10 min and the absorbance value was measured at 490 nm by using the microplate reader (Bio-Rad, Hercules, USA). The relative viability of cells was calculated as a percentage using the formula: (mean OD490 of treated cells/mean OD490 of control cells) × 100%.
Migration ability of the cells was examined by scratch assay. Transfected and control cells were cultured in six-well plate. The scratch was performed with a pipette tip when cell density reached to 80%. Once scratch was made, cells were gently washed by PBS twice, following cultured with serum-free medium. Images were captured immediately, and 24 h after the scratch was made. The cell migration distance was measured by HMIAS - 2000 software. The migration ability of cells was mirrored by relative migration ratio: Relative migration ratio = (Start distant – End distance)/Start distance.
Quantitative real-time RT-PCR and western-blot analysis
primer sequence used in the study
Forward primer (5’–3’)
Reverse primer (5’–3’)
All data were confirmed in three biological replicates. The data are expressed as the mean ± standard error. Data comparisons were conducted by using the Student’s t-test. P < 0.05 was considered to be statistically significant.
CD73 overexpression promoted proliferation and migration of cervical cancer cells
Blocking of CD73 enzyme activity could not revert its promotive effect on cells proliferation and migration
High concentration of extracellular adenosine decreased cervical cancer cells proliferation and migration
CD73 overexpression potentiated EGFR/Akt and VEGF/Akt pathway
CD73 has emerged as an important molecule during cancer growth and metastasis. This study was undertaken to evaluate the effect of CD73 on cervical cancer cells proliferation and migration. Our results showed that CD73 overexpression promoted cervical cancer cells proliferation and migration in vitro. CD73 has both enzymatic and non-enzymatic functions in cells. As a nucleotidase, CD73 catalyzes AMP breakdown into adenosine. CD73-generated adenosine has been recognized an important purinergic signaling during cancer progression, which can suppress immune response and assist tumor immune evasion in vivo via multiple pathway . However, besides evading immune destruction, rapid and un-controlled proliferation of tumor cells is another key factor for cancer growth . Regard to this, recent years, researchers investigated the direct effect of extracellular adenosine on cancer cells proliferation in vitro by adding adenosine into culture medium, these data, including ours here, have shown that high concentration of adenosine can inhibit tumor cells proliferation and migration [16–19]. However, this inhibiting effect of high concentration of adenosine is unexpected, since CD73 overexpression has been found to promote tumor cells proliferation and migration in vitro.
At first sight, the promotive effect of CD73 and inhibiting effect of adenosine on cervical cancer cells proliferation and migration seemed to be paradoxical. This apparent discrepancy could be explained on the basis of the following considerations. The concentration of adenosine is determined by a complex ectoenzyme mechanism. The generation and degradation of adenosine is catalyzed by a cascade of enzyme as follows: ATP/ADP breakdown into AMP by CD39, AMP breakdown into adenosine by CD73, and adenosine breakdown into inosine by ADA and its cofactor CD26. Thus, the increased concentration of adenosine by CD73 overexpression may be not enough to inhibit the cancer cells proliferation and migration (the adenosine concentration should be > 100 μM, which can display inhibited effects). As the low concentration of adenosine has no effect on cancer cells proliferation and migration, therefore, the promotive effect of CD73 overexpression on cells proliferation and migration in vitro were independent of its enzyme activity. Indeed, here we proved this hypothesis in Hela and SiHa cells. In present study, we found that the promotive effect of CD73 overexpression on cervical cancer cells proliferation and migration were not reverted by blocking of CD73 enzyme activity. These data suggested that CD73 promoted cervical cancer cells proliferation and migration, which was dependent of its non-enzyme activity. EGFR and VEGF have been believed to promote cancer growth and metastasis [20–23]. Co-expression of CD73 and EGFR or VEGF has been found in other type of cancers [24–26]. Consistently, in this study, we found that EGFR and VEGF expression were markedly increased in CD73 transfected cervical cancer cells. Although Zhi X’s data have suggested that CD73 may promote EGFR expression though regulating some associated transcription factor, such as PPARγ . However, the relevant studies are still few to demonstrate the regulated mechanism of CD73 on expression of EGFR and VEGF. The interaction among them needs further studies. Moreover, the Akt expression, which is an important downstream molecule of EGFR and VEGF, was also significantly higher in CD73 overexpression cells than that in control cells . In the mean while, the EGFR, VEGF and Akt expression in CD73 overexpression cells were not changed in response to APCP treatment. In summary, our data suggest that CD73 overexpression promote cervical cancer cells proliferation and migration, via potentiating EGFR/Akt and VEGF/Akt pathway, independent of its enzymatic activity. These data provide a novel insight into the regulating function of CD73 in cancer and suggest that CD73 may be promising therapeutic target in cervical cancer.
Our data showed that CD73 promoted cervical cells proliferation and migration, and this promotive effect was independent of CD73 enzyme activity. In addition, we also showed that CD73 overexpression potentiated EGFR/Akt and VEGF/Akt pathway. These data provide a novel insight into the regulating function of CD73 in cancer cells and suggest that CD73 may be promising therapeutic target in cervical cancer.
American Type Culture Collection
Fetal bovine serum
High performance liquid chromatography
Human papilloma virus
Quantitative real-time polymerase chain reaction
This work is supported by the National Natural Science Fund of China (No. 81172148).
Availability of data and materials
ZWG and HP. Wang designed the experiments with help from KD. HPW, FL, ML and XW performed the experiments. ZWG interpreted data and wrote the manuscript with help from HZZ and KD. All authors read and approved the final version of this manuscript.
The authors declare that they have no conflict of interest.
Consent for publication
Ethics approval and consent to participate
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- Gao ZW, Dong K, Zhang HZ. The roles of CD73 in cancer. Biomed Res Int. 2014;2014:460654.PubMedPubMed CentralGoogle Scholar
- Antonioli L, Hasko G, Fornai M, Colucci R, Blandizzi C. Adenosine pathway and cancer. where do we go from here? Expert Opin Ther Targets. 2014;18(9):973–7.View ArticlePubMedGoogle Scholar
- Zhang B. CD73 promotes tumor growth and metastasis. Oncoimmunology. 2012;1(1):67–70.View ArticlePubMedPubMed CentralGoogle Scholar
- Sadej R, Skladanowski AC. Dual, enzymatic and non-enzymatic, function of ecto-5’-nucleotidase (eN, CD73) in migration and invasion of A375 melanoma cells. Acta Biochim Pol. 2012;59(4):647–52.PubMedGoogle Scholar
- Antonioli L, Blandizzi C, Pacher P, Hasko G. Immunity, inflammation and cancer: a leading role for adenosine. Nat Rev Cancer. 2013;13(12):842–57.View ArticlePubMedGoogle Scholar
- Wang L, Zhou X, Zhou T, Ma D, Chen S, Zhi X, Yin L, Shao Z, Ou Z, Zhou P. Ecto-5’-nucleotidase promotes invasion, migration and adhesion of human breast cancer cells. J Cancer Res Clin Oncol. 2008;134(3):365–72.View ArticlePubMedGoogle Scholar
- Terp MG, Olesen KA, Arnspang EC, Lund RR, Lagerholm BC, Ditzel HJ, Leth-Larsen R. Anti-human CD73 monoclonal antibody inhibits metastasis formation in human breast cancer by inducing clustering and internalization of CD73 expressed on the surface of cancer cells. J Immunol. 2013;191(8):4165–73.View ArticlePubMedGoogle Scholar
- Bavaresco L, Bernardi A, Braganhol E, Cappellari AR, Rockenbach L, Farias PF, Wink MR, Delgado-Canedo A, Battastini AM. The role of ecto-5’-nucleotidase/CD73 in glioma cell line proliferation. Mol Cell Biochem. 2008;319(1–2):61–8.View ArticlePubMedGoogle Scholar
- Zhou P, Zhi X, Zhou T, Chen S, Li X, Wang L, Yin L, Shao Z, Ou Z. Overexpression of Ecto-5’-nucleotidase (CD73) promotes T-47D human breast cancer cells invasion and adhesion to extracellular matrix. Cancer Biol Ther. 2007;6(3):426–31.View ArticlePubMedGoogle Scholar
- Zhi X, Chen S, Zhou P, Shao Z, Wang L, Ou Z, Yin L. RNA interference of ecto-5’-nucleotidase (CD73) inhibits human breast cancer cell growth and invasion. Clin Exp Metastasis. 2007;24(6):439–48.View ArticlePubMedGoogle Scholar
- Mello Pde A, Filippi-Chiela EC, Nascimento J, Beckenkamp A, Santana DB, Kipper F, Casali EA, Nejar Bruno A, Paccez JD, Zerbini LF, et al. Adenosine uptake is the major effector of extracellular ATP toxicity in human cervical cancer cells. Mol Bio Cell.2014;25(19):2905–18.
- Rosa MI, Medeiros LR, Rosa DD, Bozzeti MC, Silva FR, Silva BR. Human papillomavirus and cervical neoplasia. Cad Saude Publica. 2009;25(5):953–64.View ArticlePubMedGoogle Scholar
- Luhn P, Walker J, Schiffman M, Zuna RE, Dunn ST, Gold MA, Smith K, Mathews C, Allen RA, Zhang R, et al. The role of co-factors in the progression from human papillomavirus infection to cervical cancer. Gynecol Oncol. 2013;128(2):265–70.View ArticlePubMedGoogle Scholar
- Deng B, Zhang S, Miao Y, Zhang Y, Wen F, Guo K. Down-regulation of Frizzled-7 expression inhibits migration, invasion, and epithelial-mesenchymal transition of cervical cancer cell lines. Med Oncol (Northwood, London, England). 2015;32(4):102.View ArticleGoogle Scholar
- Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell. 2011;144(5):646–74.View ArticlePubMedGoogle Scholar
- Yang D, Song J, Wu L, Ma Y, Song C, Dovat S, Nishizaki T, Liu J. Induction of senescence by adenosine suppressing the growth of lung cancer cells. Biochem Biophys Res Commun. 2013;440(1):62–7.View ArticlePubMedGoogle Scholar
- Shirali S, Aghaei M, Shabani M, Fathi M, Sohrabi M, Moeinifard M. Adenosine induces cell cycle arrest and apoptosis via cyclinD1/Cdk4 and Bcl-2/Bax pathways in human ovarian cancer cell line OVCAR-3. Tumour Biol. 2013;34(2):1085–95.View ArticlePubMedGoogle Scholar
- Aghaei M, Karami-Tehrani F, Panjehpour M, Salami S, Fallahian F. Adenosine induces cell-cycle arrest and apoptosis in androgen-dependent and -independent prostate cancer cell lines, LNcap-FGC-10, DU-145, and PC3. Prostate. 2012;72(4):361–75.View ArticlePubMedGoogle Scholar
- Virtanen SS, Kukkonen-Macchi A, Vainio M, Elima K, Harkonen PL, Jalkanen S, Yegutkin GG. Adenosine inhibits tumor cell invasion via receptor-independent mechanisms. Mol Cancer Res. 2014;12(12):1863–74.View ArticlePubMedGoogle Scholar
- Zahonero C, Sanchez-Gomez P. EGFR-dependent mechanisms in glioblastoma: towards a better therapeutic strategy. Cell Mol Life Sci. 2014;71(18):3465–88.View ArticlePubMedGoogle Scholar
- Yang R, Wu Y, Wang M, Sun Z, Zou J, Zhang Y, Cui H. HDAC9 promotes glioblastoma growth via TAZ-mediated EGFR pathway activation. Oncotarget. 2015;6(10):7644–56.View ArticlePubMedPubMed CentralGoogle Scholar
- Qi L, Xing LN, Wei X, Song SG. Effects of VEGF suppression by small hairpin RNA interference combined with radiotherapy on the growth of cervical cancer. Genet Mol Res. 2014;13(3):5094–106.View ArticlePubMedGoogle Scholar
- Wang Q, Wang S, Sun SQ, Cheng ZH, Zhang Y, Chen G, Gu M, Yao HJ, Wang Z, Zhou J, et al. The effects of RNA interference mediated VEGF gene silencing on biological behavior of renal cell carcinoma and transplanted renal tumor in nude mice. Cancer Biomark : section A of Dis markers. 2016;16(1):1–9.View ArticleGoogle Scholar
- Zhi X, Wang Y, Yu J, Yu J, Zhang L, Yin L, Zhou P. Potential prognostic biomarker CD73 regulates epidermal growth factor receptor expression in human breast cancer. IUBMB Life. 2012;64(11):911–20.View ArticlePubMedGoogle Scholar
- Wu R, Chen Y, Li F, Li W, Zhou H, Yang Y, Pei Z. Effects of CD73 on human colorectal cancer cell growth in vivo and in vitro. Oncol Rep. 2016;35(3):1750–6.PubMedGoogle Scholar
- Koszalka P, Golunska M, Stanislawowski M, Urban A, Stasilojc G, Majewski M, Wierzbicki P, Skladanowski AC, Bigda J. CD73 on B16F10 melanoma cells in CD73-deficient mice promotes tumor growth, angiogenesis, neovascularization, macrophage infiltration and metastasis. Int J Biochem Cell Biol. 2015;69:1–10.View ArticlePubMedGoogle Scholar
- Ohnishi Y, Yasui H, Kakudo K, Nozaki M. Cetuximab-resistant oral squamous cell carcinoma cells become sensitive in anchorage-independent culture conditions through the activation of the EGFR/AKT pathway. Int J Oncol. 2015;47(6):2165–72.PubMedGoogle Scholar