Expression of progesterone metabolizing enzyme genes (AKR1C1, AKR1C2, AKR1C3, SRD5A1, SRD5A2) is altered in human breast carcinoma
© Lewis et al; licensee BioMed Central Ltd. 2004
Received: 07 March 2004
Accepted: 22 June 2004
Published: 22 June 2004
Recent evidence suggests that progesterone metabolites play important roles in regulating breast cancer. Previous studies have shown that tumorous tissues have higher 5α-reductase (5αR) and lower 3α-hydroxysteroid oxidoreductase (3α-HSO) and 20α-HSO activities. The resulting higher levels of 5α-reduced progesterone metabolites such as 5α-pregnane-3,20-dione (5αP) in tumorous tissue promote cell proliferation and detachment, whereas the 4-pregnene metabolites, 4-pregnen-3α-ol-20-one (3αHP) and 4-pregnen-20α-ol-3-one (20αDHP), more prominent in normal tissue, have the opposite (anti-cancer-like) effects. The aim of this study was to determine if the differences in enzyme activities between tumorous and nontumorous breast tissues are associated with differences in progesterone metabolizing enzyme gene expression.
Semi-quantitative RT-PCR was used to compare relative expression (as a ratio of 18S rRNA) of 5αR type 1 (SRD5A1), 5αR type 2 (SRD5A2), 3α-HSO type 2 (AKR1C3), 3α-HSO type 3 (AKR1C2) and 20α-HSO (AKR1C1) mRNAs in paired (tumorous and nontumorous) breast tissues from 11 patients, and unpaired tumor tissues from 17 patients and normal tissues from 10 reduction mammoplasty samples.
Expression of 5αR1 and 5αR2 in 11/11 patients was higher (mean of 4.9- and 3.5-fold, respectively; p < 0.001) in the tumor as compared to the paired normal tissues. Conversely, expression of 3α-HSO2, 3α-HSO3 and 20α-HSO was higher (2.8-, 3.9- and 4.4-fold, respectively; p < 0.001) in normal than in tumor sample. The mean tumor:normal expression ratios for 5αR1 and 5αR2 were about 35–85-fold higher than the tumor:normal expression ratios for the HSOs. Similarly, in the unmatched samples, the tumor:normal ratios for 5αR were significantly higher than the ratios for the HSOs.
The study shows changes in progesterone metabolizing enzyme gene expression in human breast carcinoma. Expression of SRD5A1 (5αR1) and SRD5A2 (5αR2) is elevated, and expression of AKR1C1 (20α-HSO), AKR1C2 (3α-HSO3) and AKR1C3 (3α-HSO2) is reduced in tumorous as compared to normal breast tissue. The changes in progesterone metabolizing enzyme expression levels help to explain the increases in mitogen/metastasis inducing 5αP and decreases in mitogen/metastasis inhibiting 3αHP progesterone metabolites found in breast tumor tissues. Understanding what causes these changes in expression could help in designing protocols to prevent or reverse the changes in progesterone metabolism associated with breast cancer.
In vitro metabolism studies have shown that mammary tissue from mouse [1, 2], rat [3–5], cat , dog [6, 7] and human [8–10] can convert progesterone to metabolites whose formation requires the action of the enzymes, 5α-reductase (5αR), 3α-hydroxysteroid oxidoreductase (3α-HSO) and 20α-HSO. Differences between normal and tumor mammary gland tissues in the progesterone metabolizing enzyme (PME) activities have been noted [2, 3, 8–10]. Our recent observations indicate that 5α-reduced metabolites (5α-pregnanes) are produced at a significantly higher rate in tumorous than in nontumorous human breast tissue, indicating increased 5αR activity in the carcinoma. Conversely, the activities of 3α-HSO and 20α-HSO were higher in histologically non-tumorous breast tissues which produce more δ-4-pregnenes .
These observations are of particular interest because the 5α-pregnanes and 4-pregnenes have been demonstrated to exhibit opposing effects associated with breast neoplasia. Specifically, exposure of human breast cell lines to 5α-pregnanes results in increased proliferation and decreased attachment , depolymerization of F-actin  and decreases in adhesion plaque-associated vinculin . In contrast, exposure to the 4-pregnenes results, in general, in opposite (anti-cancer-like) effects by causing suppression of cell proliferation and detachment [10, 11]. Additionally, specific high-affinity receptors for the 5α-pregnane, 5α-pregnane-3,20-dione (5αP) and the 4-pregnene, 3α-hydroxy-4-pregnen-20-one (3αHP), have been identified in the plasma membrane fractions but not in the intracellular compartments of breast cancer cells . The plasma membrane-associated 5αP and 3αHP receptors are distinct from each other and from the classical intracellular androgen, estrogen, progesterone and corticosteroid receptors . These results suggest important and distinct cancer regulating functions for endogenous progesterone metabolites and emphasize a potentially prominent role for the PMEs expressed in breast tissues.
We have recently also shown that 5α-reductase activity is higher, whereas 3α-HSO and 20α-HSO activities are lower in the tumorigenic cell lines, MCF-7, MDA-MB-231, and T-47D, than in the nontumorigenic, MCF-10A, cell line . The differences in enzyme activities between the tumorigenic and nontumorigenic cell lines are correlated with differences in levels of mRNA expression  of 5α-reductase type 1 (5αR1; SRD5A1), 20α-HSO (AKR1C1), 3α-HSO type 2 (3α-HSO2; AKR1C3) and 3α-HSO type 3 (3α-HSO3; AKR1C2). A recent study  also noted that 3α-HSO type 3 (AKR1C2) mRNA expression is lower in prostatic adenocarcinoma than in normal human prostate tissue.
Taken together, these findings suggest important autocrine/paracrine cancer regulating functions for progesterone metabolites and suggest regulatory roles for 5αR, 3α-HSO and 20α-HSO activities in human breast cancer progression. The etiology of PME activity changes during breast carcinogenesis is not known. The objective of the current studies was to determine if PME gene expression levels have been altered in breast tumorigenesis. To this end, reverse transcription (RT) polymerase chain reaction (PCR) was used to quantify expression levels of 5αR1, 5αR2, 20α-HSO, 3α-HSO2 and 3α-HSO3 in paired (tumorous and nontumorous) tissues from 11 patients, and unpaired tumor tissues from 17 patients and nontumorous breast tissues from 10 reduction mammoplasty samples. The results provide the first evidence that expression levels of SRD5A1/2 are elevated whereas those of AKR1-3 are markedly decreased in breast carcinoma. The findings suggest that selective changes in specific PME expression levels could lead to local increases in carcinoma-promoting 5αP and decreases in carcinoma-inhibiting 3αHP and 20αDHP in breast cancer tissue.
Patient and biopsy information from the tumors of paired (tumor and normal from the same breast) tissue samples.
Malignant lymph nodesd
RNA isolation and reverse transcription
RNA isolation and reverse transcription (RT) was as described . Briefly, the procedure was as follows. Total RNA was isolated from the tissues by homogenization in TRIzol® reagent (Invitrogen) and extraction following the manufacturer's instructions. Prior to reverse transcription, RNA was treated with DNase I for 30 minutes at 37°C using a DNA-free™ kit (Ambion). Complementary DNA (cDNA) was obtained from 2.0 μg of DNase treated total RNA using 200 U Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Invitrogen) and random hexamer primers (Invitrogen) in a total volume of 20 μl following the manufacturers instructions. To exclude any amplification of genomic DNA, all experiments included conditions in which the reverse transcriptase enzyme was omitted. In addition, no template (NT) controls were run for both the RT and PCR stages for each of the primer sets and none showed any visible PCR product.
Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR)
All PCR primers were purchased from Invitrogen Canada (Burlington, ON) and primer sequences, PCR conditions, PCR reaction kinetics and quantification were determined as previously described . The conditions were as follows: 95°C denature for 4 minutes followed by cycling, each cycle with a 20 second denature at 94°C, a 30 second anneal at 62°C, and a 30 second extension at 72°C. Cycling was followed by a final extension for 4 minutes at 72°C. For each reaction an aliquot of cDNA was amplified in a 25 μl total volume using 1.25 U of Platinum Taq DNA Polymerase (Invitrogen), 2 mM MgCl, 0.2 mM dNTP's, and 2 mM primer. Samples were amplified in separate reactions for 12 cycles with the 18S ribosomal RNA (rRNA) primers, 27 cycles with the 5αR1, 20α-HSD, 3α-HSD1, 3α-HSD2 and 3α-HSD3 primers, and 33 cycles with the 5αR2 primers. Prior studies had shown that under these cycling conditions, quantification was linear over an 8-fold range . The PCR products were separated on 9% polyacrylamide gels and visualized using ethidium bromide staining. The bands were quantified by Quantity One 4.2.1 Gel Doc Software (BioRad Laboratories). The quantity of each band is expressed as total pixel value, based on intensity and number of pixels per band. Results are given as ratios of the total pixel value of the band of interest to the total pixel value of the 18S band. Values were obtained from the average of duplicate PCRs run on the same gel and assays were repeated three times for each gene, using a fresh cDNA sample each time.
Results are given as mean ± SEM. Data were analyzed either by Student's t test for two columns or by ANOVA followed by Student-Newman-Keuls test comparing all columns. Differences were considered significant if p < 0.05.
Table 1 summarizes information on the eleven patients and the tumor portion from each paired tissue. The ages of the women at the time of surgery ranged from 34 to 75 years. The tumorous tissues were diagnosed as infiltrating ductal carcinomas of no special type. They were graded by the Scarff-Bloom-Richardson (SBR) histological grading system, whereby two carcinomas were Grade II/III and nine were III/III. Estrogen (ER) and progesterone (PR) receptor information was available for 10 of the tumor tissues. Eight tumor tissues were ER- and PR-negative, two were ER- and PR-positive, and one was ER-positive and PR-negative. ER and PR concentrations were graded as indicated in Table 1. Two of the patients had axillary lymph nodes that contained metastases. On pathological examination, tumor was found to occupy from 25% – 75% of the frozen tissue. Normal breast parenchyma occupied from 5% – 15% of the normal breast sample with the bulk of the tissue composed of fibrous tissue and fat. Lymphocyte infiltration ranged from mild to heavy in the tumor samples and was essentially absent in the normal (nontumorous) tissues.
Expression levels of progesterone metabolizing enzymes in paired tissues
Expression levels of progesterone metabolizing enzymes (standardized against 18S mRNA) in tumorous and non-tumorous (Normal) resected breast tissues from 11 patients (described in Table 1).
5αR1 (SRD5A1) a
5αR2 (SRD5A2) b
20α-HSO (AKR1C1) a
3α-HSO-3 (AKR1C2) c
3α-HSO-2 (AKR1C3) a
1.68 ± 0.06
5.51 ± 0.30
0.99 ± 0.02
1.82 ± 0.10
0.76 ± 0.05
0.79 ± 0.11
0.16 ± 0.02
0.1 ± 0.01d
1.25 ± 0.05
1.11 ± 0.09
0.20 ± 0.01
3.42 ± 0.44
0.11 ± 0.02
3.49 ± 0.03
3.77 ± 0.26
0.31 ± 0.03
3.24 ± 0.09
0.18 ± 0.02
3.81 ± 0.13
0.49 ± 0.02
0.64 ± 0.06
1.26 ± 0.08
0.48 ± 0.05
1.79 ± 0.09
4.31 ± 0.28
1.12 ± 0.05
3.78 ± 0.33
0.89 ± 0.05
4.01 ± 0.03
1.12 ± 0.20
0.88 ± 0.12
2.68 ± 0.12
0.28 ± 0.05
0.20 ± 0.02
7.41 ± 0.25
0.42 ± 0.02
7.20 ± 1.09
0.12 ± 0.01
7.77 ± 0.93
0.65 ± 0.07
0.38 ± 0.02
1.44 ± 0.10
0.92 ± 0.16
4.06 ± 0.33
6.07 ± 0.40
0.19 ± 0.03
6.94 ± 0.41
0.17 ± 0.02
6.54 ± 0.28
2.99 ± 0.17
0.66 ± 0.02
5.08 ± 0.12
0.94 ± 0.05
1.91 ± 0.15
6.04 ± 1.07
2.42 ± 0.07
5.75 ± 0.98
2.18 ± 0.10
8.31 ± 0.48
1.97 ± 0.09
0.20 ± 0.01
4.40 ± 0.46
0.26 ± 0.02
2.28 ± 0.12
4.79 ± 0.60
1.78 ± 0.06
6.01 ± 1.09
2.39 ± 0.03
6.07 ± 0.27
2.52 ± 0.15
0.58 ± 0.08
4.70 ± 0.12
0.65 ± 0.10
0.77 ± 0.02
1.51 ± 0.05
1.71 ± 0.13
1.89 ± 0.06
5.26 ± 0.88
2.83 ± 0.35
6.05 ± 0.42
0.20 ± 0.01
2.58 ± 0.12
0.38 ± 0.11
2.41 ± 0.24
4.70 ± 0.83
1.50 ± 0.16
5.59 ± 0.25
0.67 ± 0.14
6.63 ± 0.54
1.47 ± 0.10
1.08 ± 0.28
4.32 ± 0.22
0.61 ± 0.09
2.34 ± 0.14
5.50 ± 0.51
0.68 ± 0.09
4.66 ± 0.42
0.72 ± 0.14
5.25 ± 0.11
1.52 ± 0.14
1.32 ± 0.18
3.08 ± 0.26
1.22 ± 0.15
2.46 ± 0.09
6.04 ± 0.95
0.56 ± 0.07
6.32 ± 1.01
0.42 ± 0.07
5.78 ± 0.10
1.04 ± 0.08
After the completion of the study a real-time PCR unit (Rotor Gene 3000) became available and expression levels of 5αR1 and 5αR2 (with respect to 18S rRNA) were determined on the only remaining RNA samples (from paired tissues of patients #1, #7 and #8). Expression of 5αR1 by real-time measurement, was 18.5-, 1061- and 12.5-fold higher in tumor than in normal samples from #1, #7 and #8, respectively (compared to 3.3-, 22- and 8-fold higher, respectively, by the semi-quantitative method. 5αR2 expression was higher by 6.6-, 140- and 1.3-fold by real-time measurement, (compared to 1.8-, 8.8- and 1.2-fold higher by the semi-quantitative method) in tumor than normal samples of #1, #7 and #8, respectively. The real-time results confirmed the findings by the semi-quantitative method that 5αR mRNA expression levels are higher in tumor than in normal tissue. The differences between the two methods may be due to the fact that the real-time measurements are linear over a much wider range than the 8-fold limit of the semi-quantitative method. The real-time determinations suggest that the changes in progesterone metabolizing enzyme expression that accompany breast carcinoma may be even greater than indicated by the semi-quantitative results.
The unpaired tissues consisted of breast carcinomas from 17 patients and normal (reduction mammoplasty) from 10 cases. The tumor tissues came from women whose ages at the time of surgery ranged from 48 to 91 years. Four of the tumor tissues were ER- and PR-negative, ten were ER-positive and PR-positive at varying receptor levels, and three were ER-positive and PR-negative. There were no evident relationships between ER/PR status and enzyme gene expression levels. Available SBR scores were Grade I for two tissues, Grade II for two tissues and Grade III for seven tissues. No apparent relationships between SBR scores and enzyme gene expression levels were detected. Of the ten samples of normal tissue three patients were just under 20 years, five were between 24 and 35 years and two were between 47 and 53 years of age. No ER or PR data were available for these tissues.
Expression levels of progesterone metabolizing enzymes in unpaired tissues
The potential significance for breast cancer of the changes in progesterone metabolizing enzyme activities is that the metabolites appear to be directly involved in promoting or inhibiting tumor growth in this tissue. The 5α-pregnane, 5αP, which is produced at higher levels in tumorous tissues, stimulates cell proliferation and inhibits cell anchorage [10, 11]. The action of the neoplasia-promoting 5αP involves novel, specific, high-affinity receptors in the cell membrane  and results in marked alteration of the cell's cytoskeletal and adhesion complexes normally involved in cell replication and attachment . On the other hand, the 4-pregnene, 3αHP, which is produced at higher levels in the normal breast tissue, has the opposite effect by suppressing cell proliferation and detachment [10, 11] via separate and distinct membrane receptors .
Changes in enzyme activity can result from changes in the milieu in which the enzymes operate (such as temperature and pH, and concentrations of cofactors, substrates, products, competitors, ions, phospholipids and other molecules). Changes can also occur because of changes in actual enzyme amounts due to changes in the expression of the mRNA coding for the enzyme. Since the progesterone metabolizing enzyme activity studies  on tumorous and nontumorous breast tissue were carried out in vitro in identical milieus, the observed differences can be more easily ascribed to differences in enzyme amounts resulting from altered expression. A recent study on breast cell lines has shown that differences in progesterone metabolizing enzyme activity can be correlated with changes in enzyme mRNA expression . The present study indicates that changes in the mRNA expression of various progesterone metabolizing enzymes may be responsible for the previously reported  higher levels of 5α-pregnanes and lower levels of 3α- and/or 20α-hydroxy 4-pregnenes produced by tumorous human breast tissue.
The enzyme responsible for the conversion of 4-ene steroids to 5α-reduced steroids is 5α-reductase (5αR; EC 220.127.116.11). Two isoforms of 5αR have been cloned and characterized in mammals, namely type 1 (5αR1) and type 2 (5αR2) [16, 17]. 5αR1, which is encoded by the SRD5A1 gene has an optimum pH of 6–9, whereas 5αR2, encoded by the SRD5A2 gene, has an optimum pH of 5.5 . 5αR1 has been detected in various androgen-independent organs, such as the liver and brain . 5αR2 has been found predominantly in androgen-dependent organs, such as epididymis and prostate [17, 19]. Recently 5αR1 and 5αR2 have also been located in human breast carcinoma where they were studied in relation to 5α-reduction of testosterone . In the present study we observed mRNA expression for both 5αR1 and 5αR2 in all tissues examined.
The studies on the paired samples show significantly higher levels of expression of 5αR1 and 5αR2 in tumorous than in the normal (nontumorous) breast tissue biopsies (Table 2 and Fig 3). Such an increase in expression of 5αR could account for the higher levels of progesterone 5α reduction in the tumorous portion of matched breast samples that resulted in significantly higher levels of 5αP production .
In breast cell lines the higher 5αR activity level in the tumorigenic cells , appeared to be attributable to 5αR1 since its mRNA expression was several hundred fold greater than that of 5αR2; seven more PCR cycles were required for 5αR2 in order to show band intensity approximately equivalent to 5αR1. Moreover, 5αR1 mRNA expression was significantly greater, whereas 5αR2 mRNA expression was no higher, in tumorigenic than in nontumorigenic cells . Similarly, in the present studies 33 cycles were required for 5αR2 in contrast to only 26 cycles for 5αR1 indicating that mRNA levels for 5αR1 greatly exceed those of 5αR2 in human breast tissue. It was also recently demonstrated by immunohistochemistry and RT-PCR that 5αR1 is the main isoform expressed in human breast carcinoma . Together the observations provide strong evidence that 5αR1 may be the primary 5α-reductase expressed in breast tissue. However, in contrast to prostate where no differences were found in 5αR2 expression between tumor and matched normal tissue  we saw significantly higher levels of expression of 5αR2 in tumor than in normal breast tissue in both the matched and unmatched breast samples. Therefore it is still possible that changes in 5αR2 mRNA expression have some relevance to progesterone metabolism in breast cancer.
In the unmatched samples the levels of 5αR1 expression were not significantly different between normal (mostly reduction mammoplasty) and tumor tissues. Normal samples required large amounts of tissue to obtain sufficient levels of RNA for PCR analysis. Also, the unmatched tissues had been collected separately and the records do not provide information to determine if storage factors such as interval between excision and freezing may have been different. Another important factor may be the difference in age between tumor and normal samples; eight of the normal samples were derived from premenopausal women, between the ages of 19 and 35, a time when levels of steroid hormones and enzymes vary markedly during the menstrual cycle. Further investigations will be required to resolve differences in 5αR expression at different ages and different stages of the cycle.
Hydroxysteroid oxidoreductase expression
Mammalian 3α-HSO and 20α-HSO activities result in stereospecific and positional keto/hydroxy interconversions. The isozymes responsible for these catalytic actions have been cloned and they belong to the AKR1C subfamily or the AKR (aldo-keto-reductase) superfamily . Human 20α-HSO is formally AKR1C1, human 3α-HSO type 3 is formally AKR1C2 and human 3α-HSO type 2 (identical to type 5 17β-HSD) is formally AKR1C3. These human isoforms share common properties and 83 to 97% sequence homology [21, 22]. Despite their high homology, these isoforms display distinct differences in reactivity and substrate specificity and are expressed at different levels in different human tissues [23–26]. The expression of AKR1C1, AKR1C2 and AKR1C3 mRNAs has been detected in human mammary gland [24, 27–29]. AKR1C1 has predominantly 20α-HSO (but also some 3α-HSO) activity, AKR1C2 has predominantly 3α-HSO (but also some 17β-HSO and 20α-HSO) activity, and AKR1C3 has substantial 17β-HSO (and lesser 3α-HSO and 20α-HSO) activity [14, 28].
Our results show that the level of expression of these three HSO isozymes is significantly less in neoplastic than in normal (nontumorous) breast tissue from both matched and unmatched samples. On average, the level of expression of 20α-HSO and 3α-HSO3 is 4–5-fold lower and that of 3α-HSO2 is about 2.5-fold lower in tumor than in normal samples. In a preliminary report in which tumorous tissue was compared to normal tissue in 24 paired cases, Stolz and co-workers, using real-time PCR, also observed that the expression of 20α-HSO (relative to RNase P) was significantly lower (>5-fold) in the tumor portion of the majority of the cases and nearly half of these also showed decreased 3α-HSO3 expression . Their results are thus potentially similar to ours with respect to AKR1C1 and AKR1C2. Moreover, they provided evidence that the reduced AKR1C1 gene expression is associated with decreased immunohistochemical staining in tumor compared to paired normal tissue. The reduction in 3α-HSO mRNA expression may not be confined to breast cancer. In a recent study  it was demonstrated that 3α-HSO3 mRNA expression is also lower in tumor versus normal human prostate tissue samples.
The reduced expression of 3α(20α)-HSO isozymes and increased expression of 5αR isozymes in breast tumor reported here help to explain the lower and higher level of activity, respectively, of these enzymes observed in tumor versus paired normal breast tissue . Similarly, tumorigenic breast cancer cell lines (MCF-7, MDA-MB-231, T-47D) also exhibited lower 3α-HSO and 20α-HSO and higher 5αR expression than a non-tumorigenic (MCF-10A) cell line  and these differences in expression correlated with differences in respective enzyme activities . Both in the tissues  and cell lines [unpublished], the differences in activity were reflected primarily in higher amounts of 5αP and lower amounts of 3αHP and 20αDHP produced in the neoplastic versus the normal condition.
Others [14, 28–31] have considered 3α- and 20α-reduction as catabolic pathways resulting in less active (or inactive) metabolites, thereby decreasing the amount of biologically active ligand available for androgen and progesterone receptor binding, respectively. Thus reduced expression of AKR1C2 in prostate cancer has been considered to be associated with reduced conversion of 5α-dihydrotestosterone to 5α-androstane-3α,17β-diol, thereby maintaining higher levels of 5α-dihydrotestosterone which is considered to have higher growth-promoting action . Similarly, conversion of progesterone to 20αDHP has been considered a means of decreasing the local progesterone concentrations . Instead of viewing the enzymes as regulators of progesterone concentration and the progesterone metabolites as inactive by-products of this catabolism in breast tissue, we propose that the primary function of the enzymes is to regulate the relative levels of the active 5α-pregnane and 4-pregnene progesterone metabolites. At least two of these metabolites, 3αHP and 5αP, have been shown to have independent specific and opposing activity with respect to breast cell proliferation, adhesion and cell cytoskeletal components [10, 11]. In addition, 3αHP and 5αP have separate specific membrane-associated receptors on mammary cells, which are distinct from estrogen, androgen, progesterone or corticosteroid receptors . Moreover, 3αHP has been demonstrated to exhibit meiosis regulating activity in rat testis , inhibition of follicle-stimulating hormone release in pituitary cells  via nongenomic mechanisms [34–37], and analgesic  and anxiolytic  actions in the brain. It appears, therefore, appropriate to consider at least some of the progesterone metabolites produced in breast tissue as active independent hormones, potentially involved in regulating breast cancer.
5α-Reductase and reductive 3α-HSO and 20α-HSO activities provide the major (if not exclusive) catabolic pathways of progesterone in breast tissues (Figure 7). The relative activities of these enzymes will determine the ratio of cancer-promoting 5αP with respect to cancer-inhibiting 3αHP and 20αDHP concentrations. The current findings of the decline in expression of the HSO isozymes in tumor tissue explain the lower levels of the cancer-inhibiting steroids in relation to the cancer-promoting 5αP. Increased expression in 5αR mRNA would further increase the ratio in favor of cancer-promoting progesterone metabolites. We have previously suggested that the change from normal to carcinoma and/or the promotion of the carcinoma may involve an increase in the local 5α-pregnane/4-pregnene ratio, in particular the 5αP/3αHP ratio. The current findings suggest that higher levels of 5αR expression and/or lower levels of 3α- and 20α-HSO expression in the tumor (as compared to the normal breast tissue) result in increases in local concentrations of cancer-promoting, 5αP, and simultaneous decreases in actively anti-neoplastic hormones such as 3αHP. The control of the expression level of individual progesterone metabolizing enzymes might therefore provide a mechanism of controlling breast cancer by controlling local concentrations of progesterone metabolites with opposite activities.
It is known that the progesterone metabolite, 5αP, stimulates proliferation and detachment of breast cell lines, and thus potentially promotes mitogenesis and metastasis. The progesterone metabolites, 3αHP and 20αDHP, have the opposite effects by suppressing proliferation and detachment. Conversion of progesterone to 5αP is higher, whereas conversion to 3αHP and 20αDHP is lower in tumor than in normal breast tissue. Progesterone conversion to 5αP requires the action of 5αR (SRD5A) and conversion to 3αHP and 20αDHP requires the actions of AKR1C1-AKR1C3 (3α-HSO and 20α-HSO) isozymes. The results of this study show for the first time that expression of 5αR mRNA is significantly elevated whereas expression of AKR1C1-AKR1C3 mRNA is significantly decreased in tumor compared to normal breast tissue. The findings suggest that the shift in pattern of progesterone metabolism occurring in breast carcinoma may be due to altered expression levels of the progesterone metabolizing enzymes. Understanding what causes these changes in expression could help in designing protocols to prevent or reverse the changes in progesterone metabolism associated with breast cancer.
List of abbreviations
20α-dihydroprogesterone or 20α-hydroxy-4-pregnen-3-one
reverse transcription-polymerase chain reaction.
This research was supported by grants to JPW from the Susan G. Komen Breast Cancer Foundation (# BCTR00-000805 #BCTR2000805), and from Natural Sciences and Engineering Research Council (#6865-99).
- Mori M, Tamaoki BI: Formation of a steroidal allyl alcohol in the mammary glands of mice. Steroids. 1977, 29: 517-524. 10.1016/0039-128X(77)90071-X.View ArticlePubMedGoogle Scholar
- Mori M, Tamaoki BI: In vitro metabolism of progesterone in the mammary tumor and the normal mammary gland of GRS/A strain of mice and dependency of some steroid- metabolizing enzyme activities upon ovarian function. European Journal of Cancer. 1980, 16: 185-193. 10.1016/0014-2964(80)90150-4.View ArticlePubMedGoogle Scholar
- Mori M, Tominaga T, Tamaoki BI: Steroid metabolism in normal mammary gland and in the dimethylbenzanthracene-induced mammary tumor of rats. Endocrinology. 1978, 102: 1387-1397.View ArticlePubMedGoogle Scholar
- Mori M, Tominaga T, Kitamura M, Saito T, Tamaoki B: Effect of oophorectomy on progesterone metabolism in DMBA-induced mammary tumours of the rat. European Journal of Cancer. 1980, 16: 1373-1375. 10.1016/0014-2964(80)90297-2.View ArticlePubMedGoogle Scholar
- Eechaute W, de Thibault de Boesinghe L, Lacroix E: Steroid metabolism and steroid receptors in dimethylbenz(a)anthracene- induced rat mammary tumors. Cancer Res 1983, 43: 4260-4265. Cancer Research. 1983, 43: 4260-4265.PubMedGoogle Scholar
- Shiota K, Sasaki N, Hattori N, Miura R, Hasegawa T, Guang JX, Noda K, Takahashi M: Cytosolic 20α-hydroxysteroid dehydrogenase activity in spontaneous neoplasms in the dog and cat. Journal of Veterinary Medical Science. 1991, 53: 549-552.View ArticlePubMedGoogle Scholar
- Tanaka NM, Shiota K, Noda K, Kokubo S, Kadosawa T, Nishimura R, Takahashi M, Sasaki N: 20α-Hydroxysteroid dehydrogenase activity in canine spontaneous neoplasms. Journal of Veterinary Medical Science. 1994, 56: 581-583.View ArticlePubMedGoogle Scholar
- Verma U, Kapur MM, Laumas KR: Characterization of progesterone receptors and metabolism of progesterone in the normal and cancerous human mammary gland. Journal of Steroid Biochemistry. 1978, 9: 569-577. 10.1016/0022-4731(78)90124-3.View ArticlePubMedGoogle Scholar
- Lloyd RV: Studies on the progesterone receptor content and steroid metabolism in normal and pathological human breast tissues. Journal of Clinical Endocrinology and Metabolism. 1979, 48: 585-593.View ArticlePubMedGoogle Scholar
- Wiebe John P., Muzia David, Hu Juncai, Szwajcer David, Hill Scott A., Seachrist Jennifer L.: The 4-pregnene and 5α-pregnane progesterone metabolites formed in nontumorous and tumorous breast tissue have opposite effects on breast cell proliferation and adhesion. Cancer Research. 2000, 60: 936-943.PubMedGoogle Scholar
- Wiebe John P., Muzia D: The endogenous progesterone metabolite, 5α-pregnane-3,20-dione, decreases cell-substrate attachment, adhesion plaques, vinculin expression, and polymerized F-actin in MCF-7 breast cancer cells. Endocrine. 2001, 16: 7-14. 10.1385/ENDO:16:1:07.View ArticlePubMedGoogle Scholar
- Weiler Peter J., Wiebe John P.: Plasma membrane receptors for the cancer-regulating progesterone metabolites, 5α-pregnane-3,20-dione and 3α-hydroxy-4-pregnen-20- one in MCF-7 breast cancer cells. Biochemical and Biophysical Resarch Communications. 2000, 272: 731-737. 10.1006/bbrc.2000.2847.View ArticleGoogle Scholar
- Wiebe John P., Lewis Michael J.: Activity and expression of progesterone metabolizing 5α-reductase, 20α-hydroxysteroid oxidoreductase and 3α (b)-hydroxysteroid oxidoreductases in tumorigenic (MCF-7, MDA-MB-231, T-47D) and nontumorigenic (MCF-10A) human breast cancer cells. BMC Cancer. 2003, 3: 1-15. 10.1186/1471-2407-3-9.View ArticleGoogle Scholar
- Ji Q, Chang L, VanDen Berg D, Stanczyk FZ, Stolz A: Selective reduction of AKR1C2 in prostate cancer and its role in DHT metabolism. Prostate. 2003, 54: 275-289. 10.1002/pros.10192.View ArticlePubMedGoogle Scholar
- Horwitz KB, Pike AW, Gonzales-Aller C, Fennessey PV: Progesterone metabolism in T47Dco human breast cancer cells - II. Intracellular metabolic path of progesterone and synthetic progestins. Journal of Steroid Biochemistry. 1986, 25: 911-916. 10.1016/0022-4731(86)90323-7.View ArticlePubMedGoogle Scholar
- Andersson S, Russell DW: Structural and biochemical properties of cloned and expressed human and rat steroid 5α-reductases. Proceedings of the National Academy of Sciences USA. 1990, 87: 3640-3644.View ArticleGoogle Scholar
- Russell DW, Wilson JD: Steroid 5α-reductase: two genes/two enzymes. Annual Review of Biochemistry. 1994, 63: 25-61.View ArticlePubMedGoogle Scholar
- Russell DW, Berman DM, Bryant JT, Cala KM, Davis DL, Landrum CP, Prihoda JS, Silver RI, Thigpen AE, Wigley WC: The molecular genetics of steroid 5α-reductases. Recent Progress in Hormone Research. 1994, 49: 275-284.PubMedGoogle Scholar
- Thigpen AE, Silver RI, Guileyardo JM, Casey M,L,, McConnell JD, Russell DW: Tissue distribution and ontogeny of steroid 5α-reductase isozyme expression. Journla of Clinical Investigations. 1993, 92: 903-910.View ArticleGoogle Scholar
- Suzuki T, Darnel AD, Akahira JI, Ariga N, Ogawa S, Kaneko C, Takeyama J, Moriya T, Sasano H: 5α-reductases in human breast carcinoma: possible modulator of in situ androgenic actions. Journal of Clinical Endocrinology and Metabolism. 2001, 86: 2250-2257. 10.1210/jc.86.5.2250.PubMedGoogle Scholar
- Jin Y, Penning TM: Steroid 5α-reductases and 3α-hydroxysteroid dehydrogenases: key enzymes in androgen metabolism. Best Practice Research Clinical Endocrinology and Metabolism. 2001, 15: 79-94. 10.1053/beem.2001.0120.View ArticlePubMedGoogle Scholar
- Dufort I, Soucy P, Labrie F, Luu-The V: Molecular cloning of human type 3 3α-hydroxysteroid dehydrogenase that differs from 20α-hydroxysteroid dehydrogenase by seven amino acids. Biochemical and Biophysical Resarch Communications. 1996, 228: 474-479. 10.1006/bbrc.1996.1684.View ArticleGoogle Scholar
- Dufort I, Rheault P, Huang XF, Soucy P, Luu-The V: Characteristics of a highly labile human type 5 17β-hydroxysteroid dehydrogenase. Endocrinology. 1999, 140: 568-574. 10.1210/en.140.2.568.PubMedGoogle Scholar
- Dufort I, Labrie F, Luu-The V: Human types 1 and 3 3α-hydroxysteroid dehydrogenases: differential lability and tissue distribution. Journal of Clinical Endocrinology and Metabolism. 2001, 86: 841-846. 10.1210/jc.86.2.841.PubMedGoogle Scholar
- Stolz A, Hammond L, Lou H, Takikawa H, Ronk M, Shively JE: cDNA cloning and expression of the human hepatic bile acid-binding protein. A member of the monomeric reductase gene family. Journal of Biological Chemistry. 1993, 268: 10448-10457.PubMedGoogle Scholar
- Penning TM: Molecular endocrinology of hydroxysteroid dehydrogenases. Endocrine Reviews. 1997, 18: 281-305. 10.1210/er.18.3.281.PubMedGoogle Scholar
- Khanna M, Qin KN, Wang RW, Cheng KC: Substrate specificity, gene structure, and tissue-specific distribution of multiple human 3α-hydroxysteroid dehydrogenases. Journal of Biological Chemistry. 1995, 270: 20162-20168. 10.1074/jbc.270.34.20162.View ArticlePubMedGoogle Scholar
- Penning TM, Burczynski ME, Jez JM, Hung CF, Lin HK, Ma H, Moore M, Palackal N, Ratnam K: Human 3α-hydroxysteroid dehydrogenase isoforms (AKR1C1 - AKR1C4) of the aldo-keto reductase superfamily: functional plasticity and tissue distribution reveals roles in the inactivation and formation of male and female sex hormones. Biochemical Journal. 2000, 351: 67-77. 10.1042/0264-6021:3510067.PubMedPubMed CentralGoogle Scholar
- Zhang Y, Dufort I, Rheault P, Luu-The V: Characterization of a human 20α-hydroxysteroid dehydrogenase. Journal of Molecular Endocrinology. 2000, 25: 221-228. 10.1016/S0303-7207(99)00208-7.View ArticlePubMedGoogle Scholar
- Ji Q, Aoyama C, Nien Y-D, Chen P, Liu P, Stolz A: Reduced expression of human 20α-hydroxysteroid dehydrogenase (20α-HSD) in human breast cancer cases [abstract]. Endocrine Society Annual Meeting Abstracts. 2003, 2003: P2,443-Google Scholar
- Hardy DO, Ge RS, Catterall JF, Hou YT, Penning TM, Hardy MP: Identification of the oxidative 3α-hydroxysteroid dehydrogenase activity of rat Leydig cells as type II retinol dehydrogenase. Endocrinology. 2000, 141: 1608-1617. 10.1210/en.141.5.1608.PubMedGoogle Scholar
- Campbell SMC, Wiebe JP: Stimulation of spermatocyte development in prepubertal rats by the Sertoli cell steroid, 3α-hydroxy-4-pregnen-20-one. Biology of Reproduction. 1989, 40: 897-905.View ArticlePubMedGoogle Scholar
- Wood PH, Wiebe JP: Selective suppression of follicle-stimulating hormone secretion in anterior pituitary cells by the gonadal steroid, 3α-hydroxy-4-pregnen-20-one (3αHP). Endocrinology. 1989, 125: 41-48.View ArticlePubMedGoogle Scholar
- Dhanvantari S, Wiebe JP: Suppression of follicle-stimulating hormone by the gonadal- and neurosteroid 3α-hydroxy-4-pregnen-20-one involves actions at the level of the gonadotrope membrane/calcium channel. Endocrinology. 1994, 134: 371-376. 10.1210/en.134.1.371.PubMedGoogle Scholar
- Wiebe JP, Dhanvantari Savita, Watson PH, Huang Y: Suppression in gonadotropes of gonadotropin-releasing hormone-stimulated follicle-stimulating hormone release by the gonadal- and neurosteroid 3α-hydroxy-4-pregnen-20-one involves cytosolic calcium. Endocrinology. 1994, 134: 377-382. 10.1210/en.134.1.377.PubMedGoogle Scholar
- Beck Christine A., Wolfe M, Murphy LD, Wiebe JP: Acute, nongenomic actions of the neuroactive gonadal steroid, 3α-hydroxy-4-pregnen-20-one (3αHP), on FSH release in perifused rat anterior pituitary cells. Endocrine. 1997, 6: 221-229.View ArticlePubMedGoogle Scholar
- Wiebe JP: Nongenomic actions of steroids on gonadotropin release. Recent Progress in Hormone Research. 1997, 52: 71-101.PubMedGoogle Scholar
- Wiebe John P., Kavaliers Martin: Analgesic effects of the putative FSH-suppressing gonadal steroid, 3α-hydroxy-4-pregnen-20-one: possible modes of action. Brain Research. 1988, 461: 150-157. 10.1016/0006-8993(88)90733-0.View ArticlePubMedGoogle Scholar
- Kavaliers Martin, Wiebe John P., Galea LAM: Reduction of predator-induced anxiety in mice by the neurosteroid 3α-hydroxy-4-pregnen-20-one (3αHP). Brain Research. 1994, 645: 325-329. 10.1016/0006-8993(94)91667-5.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/4/27/prepub
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