Alternative dosing of dual PI3K and MEK inhibition in cancer therapy
© Jokinen et al.; licensee BioMed Central Ltd. 2012
Received: 29 March 2012
Accepted: 18 December 2012
Published: 21 December 2012
PI3K/AKT/mTOR and RAS/RAF/MEK/ERK pathways are thought to be the central transducers of oncogenic signals in solid malignancies, and there has been a lot of enthusiasm for developing inhibitors of these pathways for cancer therapy. Some preclinical models have suggested that combining inhibitors of both parallel pathways may be more efficacious, but it remains unknown whether dual inhibition with high enough concentrations of the drugs to achieve meaningful target inhibition is tolerable in a clinical setting. Furthermore, the predictive factors for dual inhibition are unknown.
Non-small cell lung cancer (NSCLC) cell lines (n=12) with the most frequent oncogenic backgrounds (K-Ras mut n=3, EGFR mut n=3, ALK translocated n=3, and triple-negative n=3) were exposed to PI3K inhibitors (ZSTK474, PI-103) or MEK inhibitor (CI-1040) alone or in combination and analysed with an MTS growth/cytotoxicity assay and statistically by combination index analysis. The activity of the intracellular signaling pathways in response to the inhibitor treatments was analysed with a western blot using phospho-specific antibodies to AKT, ERK1/2, S6, and 4E-BPI. For the differential dosing schedule experiments, additional breast and colon cancer cell lines known to be sensitive to dual inhibition were included.
Two of the 12 NSCLC cell lines tested, H3122 (ALK translocated) and H1437 (triple-negative), showed increased cytotoxicity upon dual MEK and PI3K inhibition. Furthermore, MDA-MB231 (breast) and HCT116 (colon), showed increased cytotoxicity upon dual inhibition, as in previous studies. Activation of parallel pathways in the dual inhibition-sensitive lines was also noted in response to single inhibitor treatment. Otherwise, no significant differences in downstream intracellular pathway activity (S6 and 4E-BPI) were noted between PI3K alone and dual inhibition other than the increased cytotoxicity of the latter. In the alternative dosing schedules two out of the four dual inhibition-sensitive cell lines showed similar cytotoxicity to continuous PI3K and short (15min) MEK inhibition treatment.
Therapy with a dual PI3K and MEK inhibitor combination is more efficient than either inhibitor alone in some NSCLC cell lines. Responses to dual inhibition were not associated with any specific oncogenic genotype and no other predictive factors for dual inhibition were noted. The maximal effect of the dual PI3K and MEK inhibition can be achieved with alternative dosing schedules which are potentially more tolerable clinically.
KeywordsNon-small cell lung cancer PI3K inhibition MEK inhibition
Constitutive activation of oncogenic pathways occurs in cancers with very high frequency, and this is thought to be a central factor behind the hallmarks of cancer phenotypes, such as cycle progression, inhibition of apoptosis and metabolic reprogramming. The PI3K-AKT and RAS-RAF-MEK-ERK pathways are thought to play a central role in transmitting these oncogenic signals. Frequent cancer-associated genetic alterations such as receptor mutations or amplifications, mutations in intermediate signal transducers such as Ras, Raf or PI3KCA and inactivation of certain tumor suppressors such as PTEN lead to constitutive activation of these pathways .
The high frequency of cancer-associated genetic alterations causing constitutive activation of PI3K-AKT and RAF-MEK-ERK and the addiction of cancer cells to their signals have led to enthusiasm for developing inhibitors of these pathways. In view of the central role of such pathways in transmitting upstream oncogenic signals, their inhibition could be an effective therapy for various cancer genotypes. Some cancer genotypes have been identified in preclinical studies as responders to specific inhibitors of the pathways. HER2 amplified breast cancers have been shown to respond to PI3K inhibitors , while B Raf mutant melanomas  and triple-negative breast cancers are repressed by MEK inhibitors . The effectiveness of single pathway inhibition could be suppressed by de novo dependence on multiple signaling pathways or feedback activation of other signaling pathways in response to the inhibition of a single pathway [2, 5]. This has led to studies combining PI3K or AKT and MEK inhibitors. Dual inhibition has shown increased efficiency in various cancer genotypes in pre-clinical studies [2, 4, 6, 7] and numerous early-phase clinical studies are in progress. Clinical studies have shown the simultaneous inhibition of multiple pathways to be in all probability more toxic than inhibition of a single pathway, and no optimal dose has been established.
PI3K-mTOR inhibitors may be divided into PI3K inhibitors (such as ZSTK474), dual PI3K–mTOR inhibitors (such as PI-103) and mTOR inhibitors (rapalogs). Rapalog mTOR inhibitors are known to induce IRS-1-mediated, upstream feedback activation of PI3K-AKT , which is thought to be important for the limited clinical efficiency of the therapy for most cancers, including NSCLC. PI3K and PI3K/mTOR inhibitors should lack such feedback activation and theoretically be more active. Numerous early phase clinical trials are currently testing both single PI3K and dual PI3K/mTOR inhibitors, but it is unknown whether either is more efficient, although it is likely that a drug which hits multiple targets will be more toxic in a clinical setting.
Current oncological therapies have modest disease modifying effects in cases of non-small cell lung cancer (NSCLC), even though some disease subgroups responsive to targeted therapy have been identified in recent years. These include EGFR mutant (10-30% of patients) [9, 10] and ALK translocated (~5%) [11, 12], in which patients are highly responsive to EGFR or ALK tyrosine kinase inhibitors (TKI) [13, 14]. Furthermore, other major oncogenic disease subgroups include the K Ras mutant (~25% of patients), which is thought to be undruggable with currently available pharmacological agents .
We set out here to investigate dual inhibition with PI3K and MEK in non-small cell lung cancer (NSCLC) cell lines of various genotypes. Dual inhibition is shown to be a more effective form of therapy in some cell lines. This study also addresses administration schedules for the inhibitors which may prove less toxic in a clinical setting.
The cell lines used here included NSCLC lines with a K-Ras mutation (A549, H358, H441), EGFR mutation (H1975, HCC827, PC-9), ALK translocation (DFCI032, H2228, and H3122) and the triple negative genotype (A431, H1437, H1581), a basal-like breast cancer line MDA-MB231 and HCT116, a K-Ras mutant colorectal cell line. The NSCLC cell lines were kind gifts from Dr. Pasi Jänne (Dana-Farber Cancer Institute, Boston, USA), and the breast and colorectal lines from Dr. Peppi Koivunen (Oulu University, Oulu, Finland). The cell lines were cultured in RPMI-1640 supplemented with 5 or 10% fetal bovine serum and 100 IU/ml penicillin and streptomycin. All the cell culture reagents were purchased from HyClone (Logan, UT).
The following inhibitors were used: CI-1040, PI-103, ZSTK474 (Alexis Biochemicals; Lausen, Switzerland), and TAE684 (a kind gift from Dr. Nathanael Gray, Dana-Farber Cancer Institute, Boston, USA). All the inhibitors were dissolved in DMSO to a final concentration of 10mM and stored at −20°C. The drug solutions for the experiments were prepared from a 10mM stock solution immediately before use. MEK inhibitor CI-1040 (PD-184352), a specific small-molecule drug that inhibits MEK1/MEK2, is thought to act as an allosteric inhibitor of MEK, since it is known not to compete with the binding of either ATP or protein substrates. CI-1040 blocks ERK phosphorylation and inhibits the growth of multiple human tumor cell lines and tumor growth in xenograft models. It has been shown that the inhibitory effect of CI-1040 on cell growth is rapidly reversed after it is removed from the growth medium . ZSTK474 is a small-molecule PI3K inhibitor which has shown to be a potential antitumor agent against a human cancer xenograft in vivo with no toxicity to any critical organs . It inhibits all four PI3K isoforms, most strongly PI3Kδ, by competing with the binding of ATP to the ATP-binding-pocket of the protein. In addition, the molecule is significantly specific to PI3K, since even when administered at high concentrations it only weakly inhibits the mTOR complex, which contains a conserved PI3K domain . PI-103 is a pyridofuropyrimidine compound that selectively inhibits PI3Kα and mTOR signaling, prevents cell proliferation and invasion, causes G0-G1 cell cycle arrest and reduces tumor growth in glioma xenografts . The inhibitor has also shown significant antitumor potency in NSCLC cell lines .
Cytotoxicity/cell growth assay
Cells were plated onto 96-well plates with three to six parallel wells for each treatment, the experiments being replicated at least three times. The inhibitor treatments were started on the following day, and the plates were developed 72h later using an MTS reagent mix ([3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt], Promega; Madison, WI) supplemented with phenazine methosulfate (Sigma-Aldrich; St. Louis, MO) according to the manufacturer’s guidelines. The absorbances were read on a plate reader (Athos Labtec Instruments; Salzburg, Austria) at a wavelength of 488nm. The data were displayed graphically using GraphPad Prism (GraphPad Software; La Jolla, CA), with the absorbance in the non-treated wells as the reference value (100%). The combination index (CI) was calculated using Calcusyn software (BIOSOFT, Cambridge, UK), and a 3.3:1 ratio of the PI3K inhibitors to the MEK inhibitor was used in the CI analysis. CI values at ED50 are presented.
Western blot analysis
The cells were plated onto 6-well plates and treated with the drugs 24-48h later for 6 or 72 h, after which they were lysed in RIPA buffer (1% Igepal CA-630, 20 mM Tris–HCl pH 8.0, 137 mM NaCl, 10% glycerol, 2 mM EDTA, 1 mM sodium orthovanadate, 10 μg/mL Aprotinin, 10 μg/mL Leupeptin, and 10 μg/mL Pepstatin). Protein concentrations were measured using the Bio-Rad Protein Assay (Bio-Rad; Hercules, CA) and the concentrations in individual samples were equalized before adding 3x Laemmli buffer to a final concentration of 1x. Equal amounts of protein were run on 7.5% SDS-PAGE gels, transferred to PVDF membranes, probed with the antibodies and developed using the ECL chemiluminescence system (Millipore; Billerica, MA) for detection on radiographic films, which were scanned to an electronic format. All the antibodies used were from Cell Signaling Technologies (Danvers, MA): pAKT (S473), AKT, pERK (T202/Y204), ERK, pS6 (Thr389), S6, p4E-BP1 (Thr37/46), 4E-BP1, cleaved PARP. Anti-rabbit HRP conjugated antibody was used as a secondary antibody.
The PathScan analysis was carried out with the PathScan® RTK Signaling Antibody Array kit (Cell Signaling Technologies, Danvers, MA) according to the manufacturer’s guidelines. In brief, cells were plated on plates of diameter 6 cm and drugged the following day for 24 h. Whole cell lysates were collected, protein concentrations were determined using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA) and the protein concentrations were equalized. The lysates were applied to nitrocellulose membranes and incubated over night, washed, exposed to the secondary antibodies, developed with ECL and imaged with a Fujifilm LAS-3000 Luminescent Image analyzer and the ImageReader LAS-3000 program. The array target map can be found through the manufacturer’s homepage (http://www.cellsignal.com/products/7982.html).
Dual inhibition of PI3K and MEK in cancer cell lines
Combination index analysis
Effect of dual inhibition on cell signaling
The NSCLC (H3122 and H1437), breast cancer (MDA-MB231) and colon cancer (HCT116) lines, which showing major synergy upon dual inhibition, were further studied for cell signaling in response to the inhibitors. All the cell lines downregulated pAKT and its downstream target pS6 completely in response to 6h of treatment with the PI3K inhibitor ZSTK474 or PI-103 (3.3 μM) (Figure 3A). Downregulation of p4E-BP1 was also noted with all the cell lines tested, but it was complete only in the H3122 cell line (Figure 3A). Furthermore, concurrent activation of pERK1/2 was recognized in the H3122, MDA-MB231 and HCT116 cell lines during PI3K inhibitor treatment (Figure 3A). When the cell lines were treated with the MEK inhibitor CI-1040 (1 μM, 6 h), complete (H3122, H1437) or marked (MDA-MB231, HCT116) downregulation of pERK1/2 was seen (Figure 3A). This was accompanied by upregulation of pAKT in the H3122 and MDA-MB231 lines, but not by upregulation of pS6 or p4E-BP1 (H3122) (Figure 3A). p4E-BP1 was markedly upregulated in the MDA-MB231 line in response to CI-1040 treatment (Figure 3A).
When the PI3K and MEK inhibitors were administered simultaneously the inhibition of the targets was similar to that seen with single inhibitor treatment (Figure 3A). Dual inhibition was able to overcome the single inhibitor-induced stimulation of parallel pathway activation (Figure 3A). We were not able to detect any significant difference in the activity of either pS6 or p4E-BP1 following dual inhibitor treatment as compared with the single PI3K inhibitor treatments (Figure 3A).
Alternative dosing of dual inhibition
Since the results showed that dual inhibition needed to be administered for longer periods of time for maximal cytotoxicity, we turned next to investigating whether both inhibitors are required throughout the period of exposure. The dual inhibition-sensitive cell lines were exposed to one inhibitor throughout the treatment period (72 h) while the other inhibitor was administered concurrently for 15 min, 4 h or 24 h at the beginning of the drug exposure. The results varied significantly between the cell lines tested. In the H1437 and MDA-MB231 lines concurrent inhibition of PI3K and MEK for 15 min with continued PI3K inhibition for 72 h achieved similar cytotoxicity to concurrent inhibition for 72 h (Figure 6). Conversely, when these lines were exposed to the MEK inhibitor throughout the treatment period, short concurrent exposures (15 min, 4 h or 24 h) to PI3K inhibitors did not induce any comparable cytotoxicity (Figure 6). On the other hand, the effects of dual inhibition with PI-103 occurred faster in the H1437 line than with ZSTK474, since shorter exposures to the drug (24 h) seemed to be sufficient for maximal cytotoxicity as compared with 72h of ZSTK474 (Figure 6). In the case of the H3122 and HCT116 lines, both the PI3K and MEK inhibitors needed to be administered throughout the treatment period for maximal cytotoxicity (Figure 6).
We next investigated alternative dosing of the dual inhibition of cell signaling. The dual inhibition-sensitive lines were exposed to the PI3K inhibitors and MEK inhibitor concurrently for 15 min, after which treatment was continued with a single inhibitor for the remainder of the 6 h period. pAKT downregulation was complete or nearly complete when the cells were treated for only 15 min and with PI3K inhibitors for 6 h (Figure 3B), while conversely, pERK1/2 recovered completely in 6 h when the cells were treated with the MEK inhibitor for 15 min (Figure 3B). Interestingly, we were able to see some recovery in the activity of the downstream targets of AKT when the PI3K inhibitors were administered for 15min despite the remaining pAKT downregulation. The pS6 signal was able to recovery in the MDA-MB231 (with ZSTK474) and HCT116 (with both PI3K inhibitors) lines after short PI3K administration (Figure 3B). Furthermore, p4E-BP1 recovery was noted in the H3122 (with ZSTK474), MDA-MB231 (with ZSTK474), and HCT116 (with both PI3K inhibitors) lines (Figure 3B). Interestingly, MEK inhibitor treatment induced upregulation of p4E-BP1 in the MDA-MB231 line (Figure 3A), and marked downregulation p4E-BP1 was noted only with PI-103 (PI3K and mTOR inhibitor) in the alternative dosing experiments, but not with ZSTK474 (with a PI3K inhibitor alone) (Figure 5), suggesting mTOR-mediated activation of 4E-BP1 in response to MEK inhibition. TAE684, an ALK inhibitor, treatment was also included in the experiments conducted with the H3122 line, and this induced comparable pAKT, pERK1/2, and pS6 downregulation to that achieved with dual inhibition, whereas no change in p4E-BPI was noted (Figure 3B). Some recovery of pAKT and pS6 was seen after a short treatment with TAE684 (Figure 3B).
We went on further to analyze whether the alternative dosing could also result in apoptosis in the H3122 cell line, the only line identified as inducing apoptosis in response to dual inhibition. When the cells was treated for 15 min with dual inhibition and treatment with either the PI3K inhibitors or the MEK inhibitor was continued for 48 h, marked PARP cleavage was seen in all the treatments (Figure 4B). Furthermore, 15 min treatment with an ALK inhibitor resulted in marked PARP cleavage (Figure 4B). Cleaved PARP results were further verified with western blot analysis for cleaved caspase-3, another marker for apoptosis. Cleaved caspase-3 was detected with concurrent PI3K and MEK, or ALK inhibition while no signal was seen in PI3K or MEK inhibitor treatments. Conversely to cleaved PARP, the cleaved caspase-3 signal was much lower in alternative dosing schedules compared to continuous, concurrent PI3K and MEK inhibition (Figure 4C).
The PI3K-AKT and RAS-RAF-MEK-ERK signaling pathways are thought to be the central mediators of oncogenic signals in solid malignancies. Multiple inhibitors targeting PI3K, AKT, RAF and MEK are under development for cancer therapy, but early-phase clinical trials suggest that the single agent efficiency of such inhibitors seems to be limited, except in the case of the Raf mutant melanoma, where both RAF and MEK inhibitors have high clinical activity. There is preclinical evidence that combining the inhibitors of both pathways provides more efficient cancer therapy [2, 4, 6, 7], and some early-phase clinical trials are under way to test this approach.
We investigated here the dual pharmacological inhibition of PI3K and MEK in NSCLC cell line models with specific oncogenic genotypes. All the cell lines tested were highly responsive to single-agent PI3K inhibitors, showing a strong correlation with maximal target inhibition. This suggests that the PI3K-AKT pathway has a central role in transmitting oncogenic signals from various upstream sources, and therefore the responses to pathway inhibition are not limited to any specific cancer genotype. Furthermore, the data suggest a central role for pathway activation in the proliferation of carcinomas. The cytotoxicity of PI3K inhibitors seemed to be comparable when a PI3K (ZSTK474) or PI3K/mTOR (PI-103) inhibitors alone were used, suggesting that only PI3K inhibition matters for cytotoxicity, as administration of the MEK inhibitor seemed to have limited activity or none at all in the models tested. Two out of the twelve cell lines tested showed significantly increased cytotoxicity in response to the concurrent administration of PI3K and MEK inhibitors. Analogously to previous studies, the activity of dual inhibition was not associated with any specific oncogenic genotype, since ALK translocation-positive (H3122) and triple-negative (H1437) cell lines were the most responsive ones . In MEK inhibition-sensitive models. such as triple-negative breast or K Ras mutant colorectal cancers have shown additive cytotoxicity or reversal of resistance when MEK inhibitors have been combined with inhibitors of the PI3K-AKT-mTOR pathway [4, 7]. It is interesting to note that the dual inhibition-sensitive NSCLC lines identified here showed some cytotoxicity in response to low concentrations of MEK inhibitors (<1 μM), thereby differing from the other lines tested, which showed no response or a response only to high concentrations of the inhibitor. Furthermore, the K Ras, EGFR and ALK wild-type cell H1437 is of a rare oncogenic genotype, a MEK1 mutant, and has previously been identified as being sensitive to MEK inhibitor treatment alone . Based on the current data and previously reported findings, one could speculate that dual PI3K and MEK inhibition therapy could be the most efficient for cancers that show some dependence on MEK signaling for their proliferation or survival. Mechanistically, sensitivity to dual PI3K and MEK inhibition remains to be elucidated. It is likely that the responses are not associated with any specific oncogenic genotype but rather with inhibition of the effects of feedback activation induced by the inhibition of one pathway on the other. If this also holds good in vivo, it is likely to make the selection of patients for such treatment difficult, since no predictive biomarkers of feedback activation exist.
Even though dual inhibition of PI3K-AKT and MEK has been identified as an effective cancer therapy in preclinical models, it questionable whether this therapy is tolerable in a clinical setting concentrations high enough to achieve sufficient target inhibition. Early-phase clinical trials are in progress to test different doses and dosing schedules, but the optimal administration for maximal efficiency and tolerability remains to be elucidated. In the light of recent data from the ASCO 2012 Annual Meeting, PI3K and MEK inhibitor combination treatments are now being tested in concurrent and intermittent schedules [22, 23]. The tolerability of intermittent administration may enable higher doses of the agents to be administered than with continuous concurrent treatment . The cell line model data presented here suggest that even short courses of concurrent administration can cause marked cytotoxicity and/or apoptosis. Two out of the four dual inhibition-sensitive cell lines showed comparable cytotoxicity to that achieved with continuous administration of dual inhibition when the MEK inhibitor was administered for short periods (15 min) in combination with continuous PI3K inhibitor treatment. The increased cytotoxicity occurred even though the effects of the MEK inhibitor were quickly reversed (<6 h) after wash-out of the drug. Meanwhile H3122, an ALK translocated cell line, showed apoptosis in response to short concurrent administration of the drugs even though longer concurrent administration led to maximal cytotoxicity. Interestingly, short courses of ALK inhibition (15 min) induced comparable cytotoxicity to long administration of either an ALK inhibitor or a dual inhibitor combination, even though the ALK inhibitor is reversible in its mode of action and some recovery of the target inhibition is known to occur within 6h. In the light of our in vitro data, one could hypothesize that even a short course of dual inhibitor administration could have similar clinical effects with better tolerability. Analogously, a recent work has shown that intermittent administration of concurrent PI3K and MEK inhibition can induce robust growth inhibition in cancer cell lines . Better alternative dosing schedules for achieving clinical tolerability could also enable the use of higher doses of the drugs, leading to stronger inhibition of the target. Short but more significant target inhibition is likely to be more efficient than sub-maximal inhibition for longer periods. Our data point to the importance of maximal inhibition of the target and a preferential role for longer PI3K-AKT pathway inhibition when dual inhibition is used. These data are based only on in vitro models, however, and correlation with the in vivo situation is not always a straightforward matter.
The interconnectivity of the PI3K-AKT-mTOR and RAS-RAF-MEK-ERK pathways makes the idea of their concurrent dual inhibition an appealing one. The present cell signaling experiments also showed high interconnectivity of these two pathways, since in many instances inhibition of one pathway resulted in concurrent feedback activation of the other. Furthermore, another MEK inhibition-induced feedback mechanism was identified in the MDA-MB231 cell line which led to the activation of 4E-BP1 independently of PI3K-AKT. Previous studies have suggested that the PI3K-AKT-mTOR and RAS-RAF-MEK-ERK pathway signals converge at 4E-BP1, and that its inhibition may be a major determinant of the efficiency of dual inhibition . Conversely, we did not find any correlation between the efficiency of dual inhibition and 4E-BP1 downregulation, since the 4E-BP1 signal correlated significantly only with PI3K-AKT-mTOR activity and cytotoxicity occurred without it being downregulated. In also, some of the treatment schedules induced marked cytotoxicity in the H3122 and MDA-MB231 cell lines without the induction of any marked 4E-BP1downregulation.
The most important findings to emerge from this investigation of the concurrent dual inhibition of PI3K and MEK for cancer therapy purposes are the fact that alternative dosing schedules result in comparable cytotoxicity to that achieved with continuous treatment schedules, and that the responses to dual inhibition can be achieved in multiple cancer genotypes. The present preclinical data may offer new leads for clinical progress towards more efficient and tolerable cancer therapies.
We wish to thank Anne Bisi for her technical assistance. This research was supported by the Cancer Society of Northern Finland, the Emil Aaltonen Foundation, the Finnish Foundation for Tuberculosis Resistance, the Finnish Oncological Society, Oulu University Hospital, the Orion-Farmos Science Foundation, and the Sigrid Juselius Foundation.
- Hanahan D, Weinberg RA: Hallmarks of cancer: the next generation. Cell. 2011, 144 (5): 646-674. 10.1016/j.cell.2011.02.013.View ArticlePubMedGoogle Scholar
- Faber AC, Li D, Song Y, Liang MC, Yeap BY, Bronson RT, Lifshits E, Chen Z, Maira SM, Garcia-Echeverria C, et al: Differential induction of apoptosis in HER2 and EGFR addicted cancers following PI3K inhibition. Proc Natl Acad Sci U S A. 2009, 106 (46): 19503-19508. 10.1073/pnas.0905056106.View ArticlePubMedPubMed CentralGoogle Scholar
- Solit DB, Garraway LA, Pratilas CA, Sawai A, Getz G, Basso A, Ye Q, Lobo JM, She Y, Osman I, et al: BRAF mutation predicts sensitivity to MEK inhibition. Nature. 2006, 439 (7074): 358-362. 10.1038/nature04304.View ArticlePubMedGoogle Scholar
- Hoeflich KP, O'Brien C, Boyd Z, Cavet G, Guerrero S, Jung K, Januario T, Savage H, Punnoose E, Truong T, et al: In vivo antitumor activity of MEK and phosphatidylinositol 3-kinase inhibitors in basal-like breast cancer models. Clin Cancer Res. 2009, 15 (14): 4649-4664. 10.1158/1078-0432.CCR-09-0317.View ArticlePubMedGoogle Scholar
- Chandarlapaty S, Sawai A, Scaltriti M, Rodrik-Outmezguine V, Grbovic-Huezo O, Serra V, Majumder PK, Baselga J, Rosen N: AKT inhibition relieves feedback suppression of receptor tyrosine kinase expression and activity. Cancer Cell. 2011, 19 (1): 58-71. 10.1016/j.ccr.2010.10.031.View ArticlePubMedPubMed CentralGoogle Scholar
- Sos ML, Fischer S, Ullrich R, Peifer M, Heuckmann JM, Koker M, Heynck S, Stuckrath I, Weiss J, Fischer F, et al: Identifying genotype-dependent efficacy of single and combined PI3K- and MAPK-pathway inhibition in cancer. Proc Natl Acad Sci U S A. 2009, 106 (43): 18351-18356. 10.1073/pnas.0907325106.View ArticlePubMedPubMed CentralGoogle Scholar
- Wee S, Jagani Z, Xiang KX, Loo A, Dorsch M, Yao YM, Sellers WR, Lengauer C, Stegmeier F: PI3K pathway activation mediates resistance to MEK inhibitors in KRAS mutant cancers. Cancer Res. 2009, 69 (10): 4286-4293. 10.1158/0008-5472.CAN-08-4765.View ArticlePubMedGoogle Scholar
- O'Reilly KE, Rojo F, She QB, Solit D, Mills GB, Smith D, Lane H, Hofmann F, Hicklin DJ, Ludwig DL, et al: mTOR inhibition induces upstream receptor tyrosine kinase signaling and activates Akt. Cancer Res. 2006, 66 (3): 1500-1508. 10.1158/0008-5472.CAN-05-2925.View ArticlePubMedPubMed CentralGoogle Scholar
- Paez JG, Janne PA, Lee JC, Tracy S, Greulich H, Gabriel S, Herman P, Kaye FJ, Lindeman N, Boggon TJ, et al: EGFR mutations in lung cancer: correlation with clinical response to gefitinib therapy. Science. 2004, 304 (5676): 1497-1500. 10.1126/science.1099314.View ArticlePubMedGoogle Scholar
- Shaw AT, Yeap BY, Mino-Kenudson M, Digumarthy SR, Costa DB, Heist RS, Solomon B, Stubbs H, Admane S, McDermott U, et al: Clinical features and outcome of patients with non-small-cell lung cancer who harbor EML4-ALK. J Clin Oncol. 2009, 27 (26): 4247-4253. 10.1200/JCO.2009.22.6993.View ArticlePubMedPubMed CentralGoogle Scholar
- Soda M, Choi YL, Enomoto M, Takada S, Yamashita Y, Ishikawa S, Fujiwara S, Watanabe H, Kurashina K, Hatanaka H, et al: Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer. Nature. 2007, 448 (7153): 561-566. 10.1038/nature05945.View ArticlePubMedGoogle Scholar
- Koivunen JP, Mermel C, Zejnullahu K, Murphy C, Lifshits E, Holmes AJ, Choi HG, Kim J, Chiang D, Thomas R, et al: EML4-ALK fusion gene and efficacy of an ALK kinase inhibitor in lung cancer. Clin Cancer Res. 2008, 14 (13): 4275-4283. 10.1158/1078-0432.CCR-08-0168.View ArticlePubMedPubMed CentralGoogle Scholar
- Mok TS, Wu YL, Thongprasert S, Yang CH, Chu DT, Saijo N, Sunpaweravong P, Han B, Margono B, Ichinose Y, et al: Gefitinib or carboplatin-paclitaxel in pulmonary adenocarcinoma. New Engl J Med. 2009, 361 (10): 947-957. 10.1056/NEJMoa0810699.View ArticlePubMedGoogle Scholar
- Kwak EL, Bang YJ, Camidge DR, Shaw AT, Solomon B, Maki RG, Ou SH, Dezube BJ, Janne PA, Costa DB, et al: Anaplastic lymphoma kinase inhibition in non-small-cell lung cancer. New Engl J Med. 2010, 363 (18): 1693-1703. 10.1056/NEJMoa1006448.View ArticlePubMedPubMed CentralGoogle Scholar
- Adjei AA: K-ras as a target for lung cancer therapy. J Thorac Oncol. 2008, 3 (6 Suppl 2): S160-S163.View ArticlePubMedGoogle Scholar
- Sebolt-Leopold JS, Dudley DT, Herrera R, Van Becelaere K, Wiland A, Gowan RC, Tecle H, Barrett SD, Bridges A, Przybranowski S, et al: Blockade of the MAP kinase pathway suppresses growth of colon tumors in vivo. Nat Med. 1999, 5 (7): 810-816. 10.1038/10533.View ArticlePubMedGoogle Scholar
- Yaguchi S, Fukui Y, Koshimizu I, Yoshimi H, Matsuno T, Gouda H, Hirono S, Yamazaki K, Yamori T: Antitumor activity of ZSTK474, a new phosphatidylinositol 3-kinase inhibitor. J Natl Cancer Inst. 2006, 98 (8): 545-556. 10.1093/jnci/djj133.View ArticlePubMedGoogle Scholar
- Kong D, Yaguchi S, Yamori T: Effect of ZSTK474, a novel phosphatidylinositol 3-kinase inhibitor, on DNA-dependent protein kinase. Biol Pharm Bull. 2009, 32 (2): 297-300. 10.1248/bpb.32.297.View ArticlePubMedGoogle Scholar
- Bagci-Onder T, Wakimoto H, Anderegg M, Cameron C, Shah K: A dual PI3K/mTOR inhibitor, PI-103, cooperates with stem cell-delivered TRAIL in experimental glioma models. Cancer Res. 2011, 71 (1): 154-163. 10.1158/0008-5472.CAN-10-1601.View ArticlePubMedGoogle Scholar
- Zou ZQ, Zhang XH, Wang F, Shen QJ, Xu J, Zhang LN, Xing WH, Zhuo RJ, Li D: A novel dual PI3Kalpha/mTOR inhibitor PI-103 with high antitumor activity in non-small cell lung cancer cells. Int J Mol Med. 2009, 24 (1): 97-101.PubMedGoogle Scholar
- Marks JL, Gong Y, Chitale D, Golas B, McLellan MD, Kasai Y, Ding L, Mardis ER, Wilson RK, Solit D, et al: Novel MEK1 mutation identified by mutational analysis of epidermal growth factor receptor signaling pathway genes in lung adenocarcinoma. Cancer Res. 2008, 68 (14): 5524-5528. 10.1158/0008-5472.CAN-08-0099.View ArticlePubMedPubMed CentralGoogle Scholar
- Bedard P, Tabernero J, Kurzrock R, Britten C, Stathis A, Perez-Garcia J, Zubel A, Le N, Carter K, Bellew K, et al: A phase lb, open-label, multicenter, dose-escalation study of the oral pan-PI3K inhibitor BKM120 in combination with the oral MEK1/2 inhibitor GSK1120212 in patients (pts) with selected advanced solid tumors. J Clin Oncol. 2012, 30 (15_Suppl 1): 3003-Google Scholar
- LoRusso P, Shapiro G, Panday S, Kwak E, Jones C, Belvin M, Musib L, de Crespigny A, McKenzie M, Gates M, et al: A first-in-human phase Ib study to evaluate the MEK inhibitor GDC-0973, combined with the pan-PI3K inhibitor GDC-0941, in patients with advanced solid tumors. J Clin Oncol. 2012, 30 (15_Suppl 1): 2566-Google Scholar
- Hoeflich KP, Merchant M, Orr C, Chan J, Den Otter D, Berry L, Kasman I, Koeppen H, Rice K, Yang NY, et al: Intermittent administration of MEK inhibitor GDC-0973 plus PI3K inhibitor GDC-0941 triggers robust apoptosis and tumor growth inhibition. Cancer Res. 2012, 72 (1): 210-219. 10.1158/0008-5472.CAN-11-1515.View ArticlePubMedGoogle Scholar
- She QB, Halilovic E, Ye Q, Zhen W, Shirasawa S, Sasazuki T, Solit DB, Rosen N: 4E-BP1 is a key effector of the oncogenic activation of the AKT and ERK signaling pathways that integrates their function in tumors. Cancer Cell. 2010, 18 (1): 39-51. 10.1016/j.ccr.2010.05.023.View ArticlePubMedPubMed CentralGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2407/12/612/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.