Figure 5

The effect of c-Met, Axl and PDGFR-α expression on the migration of bladder cancer cells. (A) TSGH8301 bladder cancer cells (2 × 105 cells/200 μl DMEM) were plated in the upper chamber in the presence of 500 nM siRNA for c-Met, Axl and PDGFR-α, respectively, and analyzed for cell migration at 36 h. The filter was stained with 2% crystal violet, and the cells migrated to the opposite side of the filter were counted. (B) Quantification of (A). Data represents mean ± SD of three experiments. * *: p < 0.01, * * *: p < 0.001, by Student's t test. (C) The inhibitory effect of siRNA on c-Met, Axl and PDGFR-α expression was analyzed by Western blot analysis. Expression of c-Met, Axl and PDGFR-α was suppressed only in those cells transfected with c-Met, Axl or PDGFR-α siRNA/shRNA. There was no reduction of c-Met, Axl or PDGFR-α proteins in cells transfected with scrambled siRNA/shRNA. Ctrl: control treatment; S: scramble siRNA.