Time after 5-aza-dC | 5-aza-dC & TSA | 5-aza-dC alone | p-value |
---|
day 0
| 1.52% | 1.18% | 0.06 |
day 2
| 1.55% | 1.52% | 0.82 |
day 4
| 2.29% | 2.32% | 0.60 |
day 6
| 2.29% | 2.37% | 0.14 |
day 8
| 2.31% | 2.38% | 0.31 |
day 10
| 2.41% | 2.39% | 0.76 |
- Global methylation levels decreased with 5-aza-dC treatment and were gradually restored. T-test p-values indicate there is no synergistic effect caused by histone acetylation in the demethylation of genomic DNA.