Fig. 5
NRAV functions as an oncogene in HCC. A The expression of NRAV in hepG2 cells was analyzed by qRT-PCR after transfection with three siRNAs or the control siRNA (si-NC). B The levels of NRAV in HuH-7 cells were analyzed by qRT-PCR after stable transfection with the NRAV overexpression or the control vector. C-F Cell proliferation was assessed by CCK-8 (C, D) and colony formation (E, F). G-H Transwell assays confirmed that the migration ability of the HCC cell line was suppressed with NRAV knockdown (G) and enhanced with NRAV overexpression (H). I-K Representative images of tumors from nude mice inoculated with HepG2 cells (NRAV knockdown or control group) and NRAV knockdown decreased tumor volume and tumor weight of nude mice. Scale bars, 1 cm. Results are presented as mean ± SD. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001