Fig. 4

Identifying NRAV. A Expression levels of 6 selected genes between tumors and peritumor tissues in TCGA database. B Overall survival of HCC patients from gepia with high or low NRAV expression levels. C The expression levels of NRAV in HCC cells (HuH-7, SKhep1, hep3B, hepG2) D. Quantitative real-time PCR was performed to examine the expression of NRAV in tumors and matched non-tumor tissues from HCC patients. E RT-PCR was performed to examine cytoplasmic or nuclear NRAV RNA levels in hepG2 cells. GAPDH was the cytoplasmic control, and U6 was the nuclear control. F The constructs were transfected into HEK293T cells for 48 h with an N-terminal Flag tag. Cell lysates were harvested and subjected to Western blotting with Flag antibody. Flag-SRSF5 served as a positive control. Results are presented as mean ± SD. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001