Fig. 4

A: H128-LM cells were injected via the right carotid artery. B: In vivo imaging of leptomeningeal metastasis in mice. C: Leptomeningeal metastasis tumour tissue, H&E staining. D-G: Volcano plot demonstrating differential gene expression, especially genes with upregulated expression, with a twofold threshold and P value < 0.05. (D): GEO database; (E): H128 cells and H128-BPM cells; (F): H128 cells and H128-LM cells; (G): H128-BPM cells and H128-LM cells. In Panels E, F, and G, there were 2 samples per group. H, I: The H128-LM and H128-BPM cell lines were passaged for three generations. Immunoblot analysis of SCL44A4, VEGFA, OCLN, IGF2, EZH2, CDH1, CDH2 and MMP9 expression in four H128 cell lines (H128, H128-Mut, H128-LM, H128-BPM); beta-actin was used as a loading control