Fig. 3From: m1A regulator-mediated methylation modification patterns correlated with autophagy to predict the prognosis of hepatocellular carcinomaHigh m1A modification inhibits HCC cell proliferation and migration. (A) ALKBH3 expression detected by western blotting (up) and quantitatively analyzed (down); (B) Levels of mRNA m1A modification in sh-ALKBH3 HepG2, sh-ALKBH3 QGY, and their corresponding control cells assessed through dot blot analysis; (C) Relative cell viability of sh-ALKBH3 HepG2, sh-ALKBH3 QGY, and their corresponding control cells; (D and E) Results of colony formation (D) and migration (E) assays to detect the effects of m1A modification on HepG2 and QGY cells; (F and G) Levels of mRNA m1A modification (F) and relative cell viability (G) of HepG2 cells transfected with vector control (PPB), ALKBH3, ALKBH3-R122S, or ALKBH3-L177A constructs for 24 h (H) Results of colony formation of HepG2 cells transfected with vector control (PPB), ALKBH3, ALKBH3-R122S, or ALKBH3-L177A. Data are presented as mean ± standard deviation from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control groupBack to article page