Table 1 Comparison of different culture conditions for the construction of cell clusters using cell lines
Culture conditions in this article | Culture conditions in references | Disadvantages | References |
|---|---|---|---|
2.5% fetal bovine serum Hypoxia (5% O2) 10 ng/mL epidermal growth factor (EGF) 10 ng/mL basic fibroblast growth factor (bFGF) Shaking Table (50 rpm/min) Cells grown in suspension | Incubated with trypsin; 10% fetal bovine serum; normoxia | Loose structure, Low adhesion | [10] |
Serum-free medium (including 20Â ng/mL EGF and 20Â ng/mL bFGF); normoxia | Loose structure, Low adhesion, Expensive | [3] | |
Ultra-Low attachment cell culture flasks; 10% fetal bovine serum; normoxia | Static cultivation, Expensive | [17] | |
The Poly-HEMA- treated six-well plates; 10% fetal bovine serum; normoxia | Static cultivation, Expensive | ||
Shaking table-based method (60Â rpm/min); 10% fetal bovine serum; normoxia | Not close to the real environment | [29] |