Fig. 7

Evaluation of retention and colonization ability of cell clusters in vivo. A Representative bioluminescence images of mice at different time points after tail vein injection with luciferase-expressing 4T1 cells. B Representative images of H&E-stained sections of mouse lungs in groups injected with single cells or cell clusters, bottom: individual metastasis areas. C Up: lungs 3 days after tail vein injection of 4T1-GFP single cells or cell clusters, down: individual metastasis areas. 4T1-GFP cell clusters arrested in the lung (green). D Up: Livers 3 days after tail vein injection of 4T1-GFP single cells or cell clusters, the white arrows point to 4T1-GFP cell clusters arrested in the Liver. down: individual metastasis areas