Fig. 3

Advantages of the in vitro CTC cluster model. A Analysis of plakoglobin expression in different cell clusters by immunofluorescence. DAPI (nuclei, blue), Plakoglobin (red). The quantitated results are shown in (B), n = 5, ***P < 0.001. C Analysis of ZO-1 expression in different cell clusters by immunofluorescence. DAPI (nuclei, blue), ZO-1(red). The quantitated results are shown in (D), n = 5, ***P < 0.001. E Representative images of the appearance of cell clusters form ultralow-attach (ULA) plate and shaker. The quantitated results are shown in (F), n = 10, ***P < 0.001. G Western blot analysis of the effect of hypoxia culture on plakoglobin, PD-L1 and HIF-1α expression