Fig. 2
Other important adhesion proteins up-regulated in cell clusters. A Western blot analysis of the protein levels of ZO-1, integrin β1, integrin β5, CEACAM6 in MCF-7 cells. B MCF-7 adherent cells (1 × 106 cells) and cell clusters (1 × 105 cells) were stained for DAPI (nuclei, blue) and ZO-1 (green) by immunofluorescence assay. The quantitated results are shown in (C), n = 5, **P < 0.01. Two tail student's t-test analysis was used to compare the statistical difference between indicated two groups. D Immunofluorescence analysis of the protein levels difference of ZO-1 in 4T1 single cells and cell clusters. DAPI (nuclei, blue), ZO-1 (red). Scale bars, 20 µm. MCF-7 adherent cells (1 × 106 cells) and cell clusters (1 × 105 cells) were stained for (E) DAPI (nuclei, blue), CEACAM 6 (red), f-actin (green) and (F) claudin5 (red) by immunofluorescence assay