Fig. 7
From: ESCO2’s oncogenic role in human tumors: a pan-cancer analysis and experimental validation
ESCO2 knockdown reduced cell proliferation, invasion, and migration in ccRCC. (A)A498 cells were transfected with si-ESCO2#1 and si-ESCO2#2, the level of ESCO2 was evaluated by qRT-PCR and Western blot. The optical density ratio of bands represent objective proteins to band of β-actin was calculated. (B) CCK8 analysis of cell viability in ESCO2-knockdown A498 cells at 0, 24, and 72 h, respectively, compared with the si‐con group. (C) EdU proliferation assay in A498 cells transduced with si-NC, si-ESCO2#1 or si-ESCO2#2. Scale bar = 500 μm. (D) Transwell and Wound Healing assay (E) revealed that ESCO2 silencing inhibited the invasion and migration ability. Scale bar = 500 μm. (F) ESCO2 silencing repressed colony formation in A498 cells. *: P < 0.05, **: P < 0.01, relative to untreated control