Fig. 4
From: UBE2C promotes the proliferation of acute myeloid leukemia cells through PI3K/AKT activation
UBE2C knockdown sensitizes ferroptosis in a proteasome-dependent manner. (A) The WB analysis detects the expression levels of GPX4 and SLC7A11 proteins in HL60 and THP-1 cells. (B) The iron assay kit determines the Fe2+ levels in HL60 and THP-1 cells. (C) The flow cytometry analysis determines ROS levels in HL60 and THP-1 cells. (D) The CCK-8 method detects the relative cell viability in HL60 and THP-1 cells after different treatments (DMSO, erastin (1 µM), ferrostatin-1 (1 µM), and erastin + ferrostatin-1). MG-132 was applied at 1 µM. Cells were treated for 48 h before analysis and data in each group was normalized against the DMSO control. ** signifies P < 0.01 comparison between sh-UBE2C and sh-UBE2C + MG-132.