Fig. 2

The podoplanin-CLEC-2 interaction promotes melanoma pulmonary metastasis and decreases mouse survival in vivo. PDPN + or PDPN- B16-F0 cells (1 × 10⁵) were injected intravenously into bone marrow transplant (BMT) WT and CLEC-2 KO mice via the tail vein. (A). Representative gross images of the metastatic lung nodules from the four groups (PDPN + and PDPN- cells injected individually into BMT WT mice and BMT CLEC-2 KO mice) are shown. (B). The numbers of metastatic nodules on the surface of the lung were analysed. Data are means ± SEMs. (C). Representative cross-sections of H&E-stained lung tissues 14 days after tumour cell injection. Arrows point to metastatic foci. Scale bar = 500 μm. (D). The numbers of metastatic nodules in each right lung section. Data are means ± SEMs. (**P < 0.01) (E). Graph showing the area covered by the tumour mass relative to the total lung area in the cross-section determined by ImageJ. Data are means ± SEMs. (***P < 0.001, ****P < 0.0001) (F). Survival rates of mice injected with PDPN + cells and PDPN- cells over time. (*P < 0.0001 by log-rank test)