Fig. 1

Variant alle frequency (VAF) of MYD88 L265P and CD79B Y196 variants in. (A) In patients the VAF of MYD88 L265P ranged from 0–1% and in controls it ranged from 0–0.20%. Cut-off value was set as 0.3%. (B) In patients the VAF of CD79B Y196 ranged from 0–0.22% and in controls it ranged from 0–0.36%. Cut-off value was set as 0.5%. X-axis shows types of FFPE tissue, being controls or PCNSL. PCNSL cases are divided based on the MYD88 or CD79B ddPCR results of the tissue samples as positive, negative, or data not available (NA). Dot sizes indicate the actual total filled droplets (mutation+/wildtype+) of each cfDNA sample, ranging from 258 to 5765 filled droplets. There are 5 samples with a total number of filled droplets < 500 which were defined as inconclusive (Table S3)